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• W2004970070 endingPage "113" @default.
• W2004970070 startingPage "107" @default.
• W2004970070 abstract "The effects of pressure on the trypsin-catalyzed hydrolyzed hydrolyses of three specific substrates, N-benzoyl-L-arginine ethyl ester (BzArgOEt), amide (BzArgNH2) and p-nitroanilide (BzArgNA), have been examined. The volume of the activation (delta V++) for kcat was -2.4 ml/mol for BzArgOEt and +3 - +6 ml/mol for BzArgNH2. Because of different rate-determining steps in the steady-state kinetics, the delta V++ value for BzArgOEt would indicate the activation volume of the deacylation step, whereas that for BzArgNH2 the delta V++ for the acylation step. The activation volumes were accounted for in terms of the difference in the mechanisms on the formation and decomposition of the tetrahedral-like intermediates during the acylation and deacylation steps. The delta V values for the formation of BzArgNH2- and thionine-trypsin complexes were several ml/mol, consistent with the fact that the main driving force of the substrate binding to this enzyme is electrostatic interaction, and in contrast to the delta V values of alpha-chymotrypsin complex formation with indole (approximately 0 ml/mol) or 2-furylacryloyl-D-tryptophan methyl ester (approximately 0 ml/mol), for which the hydrophobic interaction is the dominant force of the substrate binding. For the hydrolysis of BzArgNA, which showed a distinct substrate activation at high substrate concentrations, the pressure dependence of the four parameters, ks, Ks, (the catalytic rate and dissociation constant of the normal enzyme-substrate complex, respectively), Kss and Kss (those of the complex activated by the binding of the second substrate molecule), were measured at 1 atm and 1000 atm (25 degrees C). All of the four parameters increased with increase in pressure." @default.
• W2004970070 created "2016-06-24" @default.
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• W2004970070 date "1982-05-01" @default.
• W2004970070 modified "2023-10-14" @default.
• W2004970070 title "Pressure dependence of trypsin-catalyzed hydrolyses of specific substrates" @default.
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• W2004970070 doi "https://doi.org/10.1016/0167-4838(82)90137-6" @default.
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