FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2004993076> ?p ?o ?g. }

• W2004993076 endingPage "74" @default.
• W2004993076 startingPage "57" @default.
• W2004993076 abstract "A 2-step culture system was designed and tested for the in vitro maturation efficiency of oocytes from pre-puberty preantral follicles of FVB/N inbred mice. The following modifications were made: 1) The concentration of ITS was reduced by half in the basal MIF medium to minimize uncoordinated growth between oocyte and GC cells; 2) Heterogeneous preantral follicles were cultured in groups of 3 - 5 follicles in hanging drops of medium with reduced concentration of ITS for six days to induction follicular aggregation. This hanging drop method mimics a 3-D IVM culture system at the early stage of cultivation in which the sphere structure of each follicle is well maintained. It also enables follicles in each aggregate to communicate with each other, synchronize their growth, and thus prevent immature follicular rupture. 3) Medium was further supplemented with retinoic acid to enhance developmental capacity of meiotically arrested oocytes. After a 14-day culture in vitro, ~37% of the collected inbred preantral follicles completed nuclear maturation. Approximately 94% of the mature oocytes tested were able to be fertilized; and 77% of them developed into healthy embryos. These results demonstrate that our IVM system is reliable to produce a satisfactory number of high quality oocytes. In addition, multiple cytoplasmic parameters, including gene expression of key regulators, chromosome/spindle organization, mitochondrial proliferation and distribution, and total ATP content were explored to characterize the supportive and limiting components of our IVM system so that the culture system can be further optimized." @default.
• W2004993076 created "2016-06-24" @default.
• W2004993076 creator A5002108743 @default.
• W2004993076 creator A5033970383 @default.
• W2004993076 creator A5036543946 @default.
• W2004993076 creator A5044584808 @default.
• W2004993076 creator A5071615222 @default.
• W2004993076 creator A5088281103 @default.
• W2004993076 date "2012-01-01" @default.
• W2004993076 modified "2023-09-30" @default.
• W2004993076 title "A modified protocol for &lt;i&gt;in vitro&lt;/i&gt; maturation of mouse oocytes from secondary preantral follicles" @default.
• W2004993076 cites W1534163224 @default.
• W2004993076 cites W1893337122 @default.
• W2004993076 cites W1972324826 @default.
• W2004993076 cites W1973690432 @default.
• W2004993076 cites W1974887924 @default.
• W2004993076 cites W1980581091 @default.
• W2004993076 cites W1980617562 @default.
• W2004993076 cites W1983076004 @default.
• W2004993076 cites W1988876420 @default.
• W2004993076 cites W1989101852 @default.
• W2004993076 cites W1990082011 @default.
• W2004993076 cites W1990815117 @default.
• W2004993076 cites W1992282049 @default.
• W2004993076 cites W1992308140 @default.
• W2004993076 cites W1995057744 @default.
• W2004993076 cites W1995618372 @default.
• W2004993076 cites W2000685582 @default.
• W2004993076 cites W2002944147 @default.
• W2004993076 cites W2008039120 @default.
• W2004993076 cites W2009971819 @default.
• W2004993076 cites W2012025495 @default.
• W2004993076 cites W2015428719 @default.
• W2004993076 cites W2018759592 @default.
• W2004993076 cites W2022436116 @default.
• W2004993076 cites W2023261615 @default.
• W2004993076 cites W2028320284 @default.
• W2004993076 cites W2035421357 @default.
• W2004993076 cites W2039483600 @default.
• W2004993076 cites W2041992758 @default.
• W2004993076 cites W2046346440 @default.
• W2004993076 cites W2049005503 @default.
• W2004993076 cites W2052853635 @default.
• W2004993076 cites W2054932840 @default.
• W2004993076 cites W2057698095 @default.
• W2004993076 cites W2057733021 @default.
• W2004993076 cites W2058732512 @default.
• W2004993076 cites W2058854495 @default.
• W2004993076 cites W2067867779 @default.
• W2004993076 cites W2069471554 @default.
• W2004993076 cites W2075204347 @default.
• W2004993076 cites W2075242594 @default.
• W2004993076 cites W2085563409 @default.
• W2004993076 cites W2086454122 @default.
• W2004993076 cites W2088048329 @default.
• W2004993076 cites W2091960270 @default.
• W2004993076 cites W2092224562 @default.
• W2004993076 cites W2092586744 @default.
• W2004993076 cites W2097968907 @default.
• W2004993076 cites W2099469805 @default.
• W2004993076 cites W2103752863 @default.
• W2004993076 cites W2107889339 @default.
• W2004993076 cites W2108027775 @default.
• W2004993076 cites W2108307665 @default.
• W2004993076 cites W2108489838 @default.
• W2004993076 cites W2108751988 @default.
• W2004993076 cites W2109664810 @default.
• W2004993076 cites W2113190784 @default.
• W2004993076 cites W2114467586 @default.
• W2004993076 cites W2114634093 @default.
• W2004993076 cites W2114720475 @default.
• W2004993076 cites W2116533563 @default.
• W2004993076 cites W2126657029 @default.
• W2004993076 cites W2126722856 @default.
• W2004993076 cites W2126760626 @default.
• W2004993076 cites W2127446009 @default.
• W2004993076 cites W2128760314 @default.
• W2004993076 cites W2132363501 @default.
• W2004993076 cites W2134728059 @default.
• W2004993076 cites W2136677495 @default.
• W2004993076 cites W2139507134 @default.
• W2004993076 cites W2140708045 @default.
• W2004993076 cites W2142250901 @default.
• W2004993076 cites W2143101809 @default.
• W2004993076 cites W2151847187 @default.
• W2004993076 cites W2151877403 @default.
• W2004993076 cites W2161682861 @default.
• W2004993076 cites W2163695525 @default.
• W2004993076 cites W2165132551 @default.
• W2004993076 cites W2166881613 @default.
• W2004993076 cites W2168354816 @default.
• W2004993076 cites W4234792992 @default.
• W2004993076 cites W89770524 @default.
• W2004993076 doi "https://doi.org/10.4236/abb.2012.31010" @default.
• W2004993076 hasPublicationYear "2012" @default.
• W2004993076 type Work @default.
• W2004993076 sameAs 2004993076 @default.
• W2004993076 citedByCount "8" @default.

• next