Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005011181> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2005011181 endingPage "456a" @default.
- W2005011181 startingPage "456a" @default.
- W2005011181 abstract "Buforin II (BF2) is a 21 amino acid long antimicrobial peptide. Unlike many antimicrobial peptides that induce cell death by disrupting the cellular membrane, BF2 exhibits potent antimicrobial activity without significant membrane permeabilization. A histone derivative, BF2 is hypothesized to kill bacteria by translocating across the cell membrane and binding to nucleic acids. Its membrane-penetrating property makes it a potential model for novel drug delivery systems. Pro 11 of BF2 been shown to play an important role in membrane translocation. To investigate the role played by proline, it was replaced with alanine (P11A) or reintroduced at various locations (P11A/G7P, P11A/V12P and P11A/V15P). Changing the location of the proline residue alters the peptide's overall helicity and affects the peptide's antimicrobial activity. Lipid vesicle assays showed that an optimal amount of α-helicity appears related to translocation, as increased or decreased helicity both led to reduced translocation compared to wild type BF2. However, antimicrobial activities did not correlate clearly with translocation abilities. To better understand the antimicrobial mechanism of BF2 and the role of proline, pore formation and DNA binding were investigated. The pore-forming abilities of wild type BF2 and its proline mutants were examined with a lipid vesicle dye-leakage assay. These experiments showed that increased α-helicity correlates with peptides' increased ability to cause membrane permeabilization. A fluorescent intercalator assay was used to determine the peptides' ability to bind nucleic acids. These studies revealed that while P11A/G7P exhibits a significantly stronger DNA binding ability than wild type BF2, the other mutants have similar DNA binding abilities. Together, this data helps to explain the imperfect correlation between the peptides' respective antimicrobial activities and their abilities to translocate and sheds light on the role of proline in BF2 function." @default.
- W2005011181 created "2016-06-24" @default.
- W2005011181 creator A5063697838 @default.
- W2005011181 creator A5066925608 @default.
- W2005011181 creator A5072638212 @default.
- W2005011181 creator A5079961471 @default.
- W2005011181 date "2009-02-01" @default.
- W2005011181 modified "2023-09-29" @default.
- W2005011181 title "Investigating the Role of Proline in Buforin II Function" @default.
- W2005011181 doi "https://doi.org/10.1016/j.bpj.2008.12.2344" @default.
- W2005011181 hasPublicationYear "2009" @default.
- W2005011181 type Work @default.
- W2005011181 sameAs 2005011181 @default.
- W2005011181 citedByCount "0" @default.
- W2005011181 crossrefType "journal-article" @default.
- W2005011181 hasAuthorship W2005011181A5063697838 @default.
- W2005011181 hasAuthorship W2005011181A5066925608 @default.
- W2005011181 hasAuthorship W2005011181A5072638212 @default.
- W2005011181 hasAuthorship W2005011181A5079961471 @default.
- W2005011181 hasBestOaLocation W20050111811 @default.
- W2005011181 hasConcept C185592680 @default.
- W2005011181 hasConcept C24107716 @default.
- W2005011181 hasConcept C2779281246 @default.
- W2005011181 hasConcept C2779815552 @default.
- W2005011181 hasConcept C4937899 @default.
- W2005011181 hasConcept C515207424 @default.
- W2005011181 hasConcept C540938839 @default.
- W2005011181 hasConcept C55493867 @default.
- W2005011181 hasConcept C86803240 @default.
- W2005011181 hasConcept C89423630 @default.
- W2005011181 hasConceptScore W2005011181C185592680 @default.
- W2005011181 hasConceptScore W2005011181C24107716 @default.
- W2005011181 hasConceptScore W2005011181C2779281246 @default.
- W2005011181 hasConceptScore W2005011181C2779815552 @default.
- W2005011181 hasConceptScore W2005011181C4937899 @default.
- W2005011181 hasConceptScore W2005011181C515207424 @default.
- W2005011181 hasConceptScore W2005011181C540938839 @default.
- W2005011181 hasConceptScore W2005011181C55493867 @default.
- W2005011181 hasConceptScore W2005011181C86803240 @default.
- W2005011181 hasConceptScore W2005011181C89423630 @default.
- W2005011181 hasIssue "3" @default.
- W2005011181 hasLocation W20050111811 @default.
- W2005011181 hasOpenAccess W2005011181 @default.
- W2005011181 hasPrimaryLocation W20050111811 @default.
- W2005011181 hasRelatedWork W1984164177 @default.
- W2005011181 hasRelatedWork W2002345280 @default.
- W2005011181 hasRelatedWork W2011312293 @default.
- W2005011181 hasRelatedWork W2041969558 @default.
- W2005011181 hasRelatedWork W2043183704 @default.
- W2005011181 hasRelatedWork W2044192034 @default.
- W2005011181 hasRelatedWork W2066212204 @default.
- W2005011181 hasRelatedWork W2093823523 @default.
- W2005011181 hasRelatedWork W2097226308 @default.
- W2005011181 hasRelatedWork W2123231250 @default.
- W2005011181 hasRelatedWork W2147668388 @default.
- W2005011181 hasRelatedWork W2170381215 @default.
- W2005011181 hasRelatedWork W2339728942 @default.
- W2005011181 hasRelatedWork W2368212673 @default.
- W2005011181 hasRelatedWork W2475493999 @default.
- W2005011181 hasRelatedWork W2765719309 @default.
- W2005011181 hasRelatedWork W2971787971 @default.
- W2005011181 hasRelatedWork W3049225057 @default.
- W2005011181 hasRelatedWork W3083605604 @default.
- W2005011181 hasRelatedWork W3157522736 @default.
- W2005011181 hasVolume "96" @default.
- W2005011181 isParatext "false" @default.
- W2005011181 isRetracted "false" @default.
- W2005011181 magId "2005011181" @default.
- W2005011181 workType "article" @default.