FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005014916> ?p ?o ?g. }

• W2005014916 endingPage "190" @default.
• W2005014916 startingPage "177" @default.
• W2005014916 abstract "Nile Red is a fluorescent dye used extensively to study fat accumulation in many types of cells; unfortunately protocols that work well for most cells are not effective for studying drug-induced lipid accumulation in cultured liver cells and hepatocyte-derived cell lines. Using human hepatoma (HepG2) cells, we have developed a simple Nile Red binding assay as a screen for steatosis-inducing compounds. Increases in Nile Red binding in response to known hepatotoxic compounds were observed after incubating treated cells with 1 microM Nile Red for several hours, washing away free Nile Red, and then allowing redistribution, and/or clearance of the lipid-indicator dye. Several compounds known to cause hepatic fat accumulation in vivo were examined and most robustly increased Nile Red binding in HepG2 cells. These include estrogen and other steroids, ethionine, cyclosporin A, and valproic acid. Required concentrations for increased Nile Red binding were generally three-fold or more lower than the cytotoxic concentration determined by a resazurin reduction assay in the same cells. Qualitatively similar Nile Red binding results were obtained when primary canine or rat hepatocytes were used. Morphological differences in Nile Red staining were observed by confocal fluorescence microscopy in HepG2 cells after treatment with different compounds and likely reflect distinct toxicological mechanisms." @default.
• W2005014916 created "2016-06-24" @default.
• W2005014916 creator A5014366298 @default.
• W2005014916 creator A5018948653 @default.
• W2005014916 creator A5027475930 @default.
• W2005014916 creator A5029857264 @default.
• W2005014916 creator A5032056146 @default.
• W2005014916 creator A5038108146 @default.
• W2005014916 creator A5081351380 @default.
• W2005014916 creator A5088969490 @default.
• W2005014916 date "2001-09-01" @default.
• W2005014916 modified "2023-10-14" @default.
• W2005014916 title "Nile Red Binding to HepG2 Cells: An Improved Assay for<i>In Vitro</i>Studies of Hepatosteatosis" @default.
• W2005014916 cites W1532645188 @default.
• W2005014916 cites W1558589233 @default.
• W2005014916 cites W1875842333 @default.
• W2005014916 cites W1973437334 @default.
• W2005014916 cites W1979019344 @default.
• W2005014916 cites W1984099131 @default.
• W2005014916 cites W1986170848 @default.
• W2005014916 cites W1986631454 @default.
• W2005014916 cites W1990878648 @default.
• W2005014916 cites W1993866534 @default.
• W2005014916 cites W1994206936 @default.
• W2005014916 cites W1994874483 @default.
• W2005014916 cites W1999033808 @default.
• W2005014916 cites W2006220506 @default.
• W2005014916 cites W2008675936 @default.
• W2005014916 cites W2013341934 @default.
• W2005014916 cites W2014004316 @default.
• W2005014916 cites W2014633857 @default.
• W2005014916 cites W2018852450 @default.
• W2005014916 cites W2019257805 @default.
• W2005014916 cites W2027643680 @default.
• W2005014916 cites W2028486570 @default.
• W2005014916 cites W2032351464 @default.
• W2005014916 cites W2034029712 @default.
• W2005014916 cites W2043652485 @default.
• W2005014916 cites W2043961682 @default.
• W2005014916 cites W2053981343 @default.
• W2005014916 cites W2067985468 @default.
• W2005014916 cites W2084399397 @default.
• W2005014916 cites W2085100477 @default.
• W2005014916 cites W2093013671 @default.
• W2005014916 cites W2104049337 @default.
• W2005014916 cites W2116883856 @default.
• W2005014916 cites W2118298427 @default.
• W2005014916 cites W2134579258 @default.
• W2005014916 cites W2134886973 @default.
• W2005014916 cites W2135872454 @default.
• W2005014916 cites W2160466923 @default.
• W2005014916 cites W2331931123 @default.
• W2005014916 doi "https://doi.org/10.1089/109793301753407948" @default.
• W2005014916 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11846991" @default.
• W2005014916 hasPublicationYear "2001" @default.
• W2005014916 type Work @default.
• W2005014916 sameAs 2005014916 @default.
• W2005014916 citedByCount "93" @default.
• W2005014916 countsByYear W20050149162012 @default.
• W2005014916 countsByYear W20050149162013 @default.
• W2005014916 countsByYear W20050149162014 @default.
• W2005014916 countsByYear W20050149162015 @default.
• W2005014916 countsByYear W20050149162016 @default.
• W2005014916 countsByYear W20050149162017 @default.
• W2005014916 countsByYear W20050149162018 @default.
• W2005014916 countsByYear W20050149162019 @default.
• W2005014916 countsByYear W20050149162020 @default.
• W2005014916 countsByYear W20050149162021 @default.
• W2005014916 countsByYear W20050149162022 @default.
• W2005014916 countsByYear W20050149162023 @default.
• W2005014916 crossrefType "journal-article" @default.
• W2005014916 hasAuthorship W2005014916A5014366298 @default.
• W2005014916 hasAuthorship W2005014916A5018948653 @default.
• W2005014916 hasAuthorship W2005014916A5027475930 @default.
• W2005014916 hasAuthorship W2005014916A5029857264 @default.
• W2005014916 hasAuthorship W2005014916A5032056146 @default.
• W2005014916 hasAuthorship W2005014916A5038108146 @default.
• W2005014916 hasAuthorship W2005014916A5081351380 @default.
• W2005014916 hasAuthorship W2005014916A5088969490 @default.
• W2005014916 hasConcept C121332964 @default.
• W2005014916 hasConcept C122927707 @default.
• W2005014916 hasConcept C150903083 @default.
• W2005014916 hasConcept C153911025 @default.
• W2005014916 hasConcept C178790620 @default.
• W2005014916 hasConcept C185592680 @default.
• W2005014916 hasConcept C202751555 @default.
• W2005014916 hasConcept C207001950 @default.
• W2005014916 hasConcept C2776092724 @default.
• W2005014916 hasConcept C2776200302 @default.
• W2005014916 hasConcept C2778086801 @default.
• W2005014916 hasConcept C2779348098 @default.
• W2005014916 hasConcept C2780806160 @default.
• W2005014916 hasConcept C54355233 @default.
• W2005014916 hasConcept C55493867 @default.
• W2005014916 hasConcept C62520636 @default.
• W2005014916 hasConcept C74864618 @default.
• W2005014916 hasConcept C81885089 @default.
• W2005014916 hasConcept C86803240 @default.

• next