Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005027059> ?p ?o ?g. }
- W2005027059 endingPage "2425" @default.
- W2005027059 startingPage "2412" @default.
- W2005027059 abstract "Limbal stem cells (LSCs) derived from human embryonic stem cells (hESCs) hold great potential for cell-based therapies for ocular surface diseases. The cell-based therapies mainly depend on an appropriate differentiation proposal with a high efficiency and on a suitable scaffold. In this study, we aimed to establish a feasible and efficient strategy for inducing hESCs into LSC-like cells by the LSC conditioned medium. The induced cells possessed the similar morphologic characteristics and expression of normal LSCs and showed a strong clonogenic and proliferative capacity in vitro. To construct a tissue-engineering corneal graft, these differentiated cells were seeded on an acelluar porcine corneal matrix (APCM), which maintained the corneal basement membrane in vitro. After 14 days culture, these induced cells gave rise to stratified epithelial cell sheets on the APCM and the basal cells still kept LSC characteristics. In rabbit total limbal stem cell deficiency (LSCD) models, the tissue-engineering graft had the potential to reconstruct the damaged ocular surface and alleviated the invasion of corneal neovascularization. These findings indicated that the engraftment constructed with the APCM scaffold and hESC-derived LSCs might be a potential therapy option for ocular surface regeneration in LSCD cases." @default.
- W2005027059 created "2016-06-24" @default.
- W2005027059 creator A5003850660 @default.
- W2005027059 creator A5018713038 @default.
- W2005027059 creator A5030031217 @default.
- W2005027059 creator A5044683577 @default.
- W2005027059 creator A5056560444 @default.
- W2005027059 creator A5061015930 @default.
- W2005027059 creator A5090579541 @default.
- W2005027059 date "2013-11-01" @default.
- W2005027059 modified "2023-10-16" @default.
- W2005027059 title "Reconstruction of Functional Ocular Surface by Acellular Porcine Cornea Matrix Scaffold and Limbal Stem Cells Derived from Human Embryonic Stem Cells" @default.
- W2005027059 cites W1556766786 @default.
- W2005027059 cites W1969097782 @default.
- W2005027059 cites W1970892084 @default.
- W2005027059 cites W1972552247 @default.
- W2005027059 cites W1972609931 @default.
- W2005027059 cites W1973764628 @default.
- W2005027059 cites W1975479312 @default.
- W2005027059 cites W1977091888 @default.
- W2005027059 cites W1980901440 @default.
- W2005027059 cites W1983715643 @default.
- W2005027059 cites W1993534485 @default.
- W2005027059 cites W1995865777 @default.
- W2005027059 cites W1996124198 @default.
- W2005027059 cites W2004972310 @default.
- W2005027059 cites W2006712783 @default.
- W2005027059 cites W2012219406 @default.
- W2005027059 cites W2018738384 @default.
- W2005027059 cites W2024286953 @default.
- W2005027059 cites W2030646994 @default.
- W2005027059 cites W2036162319 @default.
- W2005027059 cites W2036607348 @default.
- W2005027059 cites W2040043194 @default.
- W2005027059 cites W2040941616 @default.
- W2005027059 cites W2042280852 @default.
- W2005027059 cites W2046603933 @default.
- W2005027059 cites W2047887312 @default.
- W2005027059 cites W2053517020 @default.
- W2005027059 cites W2062445152 @default.
- W2005027059 cites W2062830648 @default.
- W2005027059 cites W2063161074 @default.
- W2005027059 cites W2075688585 @default.
- W2005027059 cites W2081812417 @default.
- W2005027059 cites W2084872508 @default.
- W2005027059 cites W2091580452 @default.
- W2005027059 cites W2091651780 @default.
- W2005027059 cites W2100465544 @default.
- W2005027059 cites W2116520192 @default.
- W2005027059 cites W2121062346 @default.
- W2005027059 cites W2121987769 @default.
- W2005027059 cites W2122300492 @default.
- W2005027059 cites W2142911414 @default.
- W2005027059 cites W2144493317 @default.
- W2005027059 cites W2145488206 @default.
- W2005027059 cites W2164311458 @default.
- W2005027059 cites W2165365345 @default.
- W2005027059 cites W2171828750 @default.
- W2005027059 cites W4232310547 @default.
- W2005027059 doi "https://doi.org/10.1089/ten.tea.2013.0097" @default.
- W2005027059 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23675636" @default.
- W2005027059 hasPublicationYear "2013" @default.
- W2005027059 type Work @default.
- W2005027059 sameAs 2005027059 @default.
- W2005027059 citedByCount "52" @default.
- W2005027059 countsByYear W20050270592014 @default.
- W2005027059 countsByYear W20050270592015 @default.
- W2005027059 countsByYear W20050270592016 @default.
- W2005027059 countsByYear W20050270592017 @default.
- W2005027059 countsByYear W20050270592018 @default.
- W2005027059 countsByYear W20050270592019 @default.
- W2005027059 countsByYear W20050270592020 @default.
- W2005027059 countsByYear W20050270592021 @default.
- W2005027059 countsByYear W20050270592022 @default.
- W2005027059 countsByYear W20050270592023 @default.
- W2005027059 crossrefType "journal-article" @default.
- W2005027059 hasAuthorship W2005027059A5003850660 @default.
- W2005027059 hasAuthorship W2005027059A5018713038 @default.
- W2005027059 hasAuthorship W2005027059A5030031217 @default.
- W2005027059 hasAuthorship W2005027059A5044683577 @default.
- W2005027059 hasAuthorship W2005027059A5056560444 @default.
- W2005027059 hasAuthorship W2005027059A5061015930 @default.
- W2005027059 hasAuthorship W2005027059A5090579541 @default.
- W2005027059 hasConcept C104317684 @default.
- W2005027059 hasConcept C105702510 @default.
- W2005027059 hasConcept C106487976 @default.
- W2005027059 hasConcept C118487528 @default.
- W2005027059 hasConcept C136229726 @default.
- W2005027059 hasConcept C142724271 @default.
- W2005027059 hasConcept C145103041 @default.
- W2005027059 hasConcept C171056886 @default.
- W2005027059 hasConcept C185592680 @default.
- W2005027059 hasConcept C189165786 @default.
- W2005027059 hasConcept C190672674 @default.
- W2005027059 hasConcept C2776193487 @default.
- W2005027059 hasConcept C2776882836 @default.
- W2005027059 hasConcept C2778155685 @default.
- W2005027059 hasConcept C2779257441 @default.