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• W2005033393 startingPage "842" @default.
• W2005033393 abstract "Flavonoid signals from alfalfa (Medicago sativa L.) induce transcription of nodulation (nod) genes in Rhizobium meliloti. Previous investigations identified the flavone luteolin as an active inducer in alfalfa seed extracts, but the nature of nod inducers released from roots has not been reported. Root exudate from 3-day-old alfalfa seedlings was purified and then assayed for biological activity with a nodABC-lacZ fusion in R. meliloti. Indentities of major nod inducers were established by spectroscopic analyses (ultraviolet/visible, proton nuclear magnetic resonance, and mass spectroscopy) and comparison with authentic standards. Major nod inducers, which were identified as 4',7-dihydroxyflavone, 4'-7-dihydroxyflavanone, and 4,4'-dihydroxy-2'-methoxychalcone, were released from seedling roots at 54, 22, and 20 picomole.plant(-1).day(-1), respectively. Luteolin was not found in these root exudates. The 4,4'-dihydroxy-2'-methoxychalcone induced nod genes at a concentration one order of magnitude lower than luteolin and is the first naturally released chalcone reported to have this function. Moderate and weak nod-inducing activity was associated, respectively, with 4',7-dihydroxyflavone and 4',7-dihydroxyflavanone." @default.
• W2005033393 created "2016-06-24" @default.
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• W2005033393 date "1989-11-01" @default.
• W2005033393 modified "2023-10-01" @default.
• W2005033393 title "A Chalcone and Two Related Flavonoids Released from Alfalfa Roots Induce <i>nod</i> Genes of <i>Rhizobium meliloti</i>" @default.
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• W2005033393 doi "https://doi.org/10.1104/pp.91.3.842" @default.
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