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- W2005033947 abstract "A reagent 1-nitroguanyl-3,5-dimethyl pyrazole was use for nitroguanidination of bovine serum albumin. This reagent permitted extensive nitroguanidination of the free amino groups of serum albumin at pH 9.5. Tyrosine, tryptophan, and histidine residues were unaffected. The modified protein showed a slight increase in the sedimentation coefficient and a decrease in diffusion coefficient. The molecular weight determined from the sedimentation and diffusion data was 74270 which is good agreement with a theoretical value of 73500. Electrophoretically nitroguanidated serum albumin showed an increase in mobility and appeared to split into two components." @default.
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- W2005033947 date "1964-12-01" @default.
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- W2005033947 title "A new reagent for nitroguanidination of proteins" @default.
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- W2005033947 doi "https://doi.org/10.1016/0304-4165(64)90337-x" @default.
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