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• W2005044186 abstract "Chloride (Cl – ) efflux induces depolarization and contraction of vascular smooth muscle cells. In the basilar arteries from the New Zealand white rabbits, the role of Cl – flux in serotonin-induced contraction was demonstrated by (i) inhibition of Na + -K + -2Cl – co-transporter (NKCC1) to decreased Cl – influx with bumetanide; (ii) a disabled Cl – /HCO 3 – exchanger with bicarbonate free HEPES solution; (iii) blockade of Cl – channels using 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) and indanyloxyacetic acid 94, R-(+)-methylindazone (R-(+)-IAA-94); and (iv) substitution of extracellular Cl – with methanesulfonate acid (113 mmol/L; Cl – , 10 mmol/L). In addition, the expression of NKCC1 in brain tissues after neonatal hypoxia-ischemia was examined at mRNA and protein levels using RT-PCR and Western blotting techniques. NKCC1 mRNA and protein expressions were increased at 24 and 48 h and returned to normal levels at 72 h after hypoxia insult when compared with the control littermates. In conclusion, Cl – efflux regulates cerebral circulation and the up-regulation of NKCC1 after neonatal hypoxia-ischemia may contribute to brain injury. Key words: serotonin, NCCK1, neonatal hypoxia." @default.
• W2005044186 created "2016-06-24" @default.
• W2005044186 creator A5004014354 @default.
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• W2005044186 date "2005-08-01" @default.
• W2005044186 modified "2023-10-01" @default.
• W2005044186 title "Role of Cl^–in cerebral vascular tone and expression of Na⁺-K⁺-2Cl^–co-transporter after neonatal hypoxia-ischemia" @default.
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• W2005044186 doi "https://doi.org/10.1139/y05-076" @default.
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