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- W2005050526 abstract "Eleven new chemically bonded silica stationary phase materials were synthesized and characterized physicochemically in search for the separation systems suitable for analysis of natural nucleosides and nucleotides. A set of 11 fundamental nucleosides and cyclic nucleotides was analyzed in 15 reversed-phase high-performance liquid chromatographic (RP-HPLC) systems. The HPLC systems comprised additionally 4 reference columns of different chemical structure. For the test solutes 10 structural descriptors were derived by molecular modelling. Sets of retention parameters and of structural descriptors were subjected to chemometric analysis by the method of principal component analysis (PCA). It was demonstrated that of the total number of 11 newly synthesized stationary phase materials 8 hydrocarbon-bonded silica phases had separation properties regarding nucleosides and nucleotides similar to a standard octylsilica phase. The phases which comprised aromatic fragments in their ligands did not differ significantly from the aliphatic hydrocarbon-silica materials. The new phases comprising methylimidazole and to a lesser extent pyridine and cyano moiety, had specific properties regarding the separation of nucleosides and cyclic nucleotides. It has been demonstrated that PCA of structural descriptors of solutes obtained by molecular modelling facilitates identification of structural features which determine retention in individual HPLC systems." @default.
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- W2005050526 date "1996-03-01" @default.
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- W2005050526 title "New stationary phases for the high-performance liquid chromatographic separation of nucleosides and cyclic nucleotides synthesis and chemometric analysis of retention data" @default.
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- W2005050526 doi "https://doi.org/10.1016/0021-9673(95)01238-9" @default.
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