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- W2005050997 abstract "Complementary DNA (cDNA) was prepared with viral RNA-dependent DNA polymerase using human globin messenger RNA (mRNA) as template. By selective hydridization to globin mRNA from beta-thalassaemics a probe which was greater than 85% complementary to alpha-globin mRNA was purified. This was hybridized in cDNA excess to human genomic DNA, and the rate and extent of hybridization confirmed that there are two genes for alpha-globin per haploid genome. Cellular DNA was also prepared from peripheral blood from cases expressing the alpha-globin chain mutant Hb J Mexico to varying extents. This DNA was identical in hybridization behaviour to normal DNA demonstrating that the imbalanced mutant chain synthesis seen physiologically is not due to a gene deletion." @default.
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- W2005050997 date "1977-08-01" @default.
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- W2005050997 title "Demonstration of Two alpha-Globin Genes per Human Haploid Genome for Normals and Hb J Mexico" @default.
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- W2005050997 doi "https://doi.org/10.1111/j.1432-1033.1977.tb11725.x" @default.
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