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• W2005064662 abstract "Abstract Special interest has been focused on the variation in the composition and content of phospholipids (PLs) in soybeans following genetic modification, due to their beneficial effects on human health. As part of the attempt to discover PL-rich soybeans, a total of 12 cultivars were bred and their PL contents were determined by LC–MS/MS. In the LC, 0.1 M ammonium formate in methanol was essential to obtain good peak separation with minimum tailing. The LC–MS/MS method operating in the multiple reaction monitoring (MRM) mode, with two transitions monitored for each PL, provided good selectivity and sensitivity for the determination of PLs. All the calibration curves were linear, with correlation coefficients higher than 0.990. The extraction recoveries ranged from 75.6% to 89.9%. The limit of quantitations (LOQs) were within the range of 0.16–16.7 μg/kg. The average content of total PLs in the 12 soybean cultivars was about 75.7 mg/kg. The total PL and PC (phosphatidylcholine) contents were highest in the Gaechuck#2 cultivar. From the viewpoint of the PL content, Gaechuck#2 was demonstrated to be the most improved variety in the 12 cultivars investigated." @default.
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• W2005064662 date "2010-06-01" @default.
• W2005064662 modified "2023-09-25" @default.
• W2005064662 title "Determination of phospholipids in soybean (Glycine max (L.) Merr) cultivars by liquid chromatography–tandem mass spectrometry" @default.
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• W2005064662 doi "https://doi.org/10.1016/j.jfca.2009.12.015" @default.
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