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• W2005067189 abstract "When the tumor promoter, 12-0-tetradecanoyl-phorbol-13-acetate (TPA), was added to freshly seeded cultures of human diploid fibroblasts, cell growth was inhibited for 24–48 h and then proceeded at the same rate as in controls. After the control cultures had become confluent, cell division and the incorporation of tritiated thymidine continued in treated cultures, and the cell yield after 9–11 days was increased by as much as 50% compared to untreated cultures. TPA induced striking morphological changes including a decrease in cell size, suggesting that it may enhance cell division by increasing the number of cells per unit area required for cell-to-cell contact and confluence to be attained. TPA produced similar effects when added to growing cultures of NIH Swiss 3T3 mouse embryo and chick embryo fibroblasts. Continuous cultivation of human fibroblasts in the presence of TPA increased the cell yield for approximately five successive passages but did not increase the total number of population doublings over the entire life-span of the cultures." @default.
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• W2005067189 date "1974-05-15" @default.
• W2005067189 modified "2023-09-28" @default.
• W2005067189 title "Growth stimulation of human diploid fibro-blasts by the tumor promoter, 12-0-tetradecanoyl-phorbol-13-acetate" @default.
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• W2005067189 doi "https://doi.org/10.1002/ijc.2910130516" @default.
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