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- W2005126370 abstract "A three dimensional (3D) matrices for cell culture is widely used in the cell engineering field. In the living organisms, the cells react to the extra cellular matrix such as collagen. Thus, the biological interaction between the extra cellular environment and cell function is required to be elucidated in order to design an ideal environment for cell culture. In this study, cytocompatible phospholipid polymer hydrogels were prepared to encapsulate cells and the physiochemical properties of the cell-encapsulated hydrogel were investigated. The hydrogels were prepared using poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-vinylphenylboronic acid) (PMBV). Mixture of PMBV aq. solution and poly(vinyl alcohol) (PVA) aq. solution under the biological condition spontaneously forms the PMBV/PVA hydrogels. Storage modulus of the hydrogels was tuned through adjustment of the mixing ratio of the polymer solution. An epidermal cells were encapsulated in the PMBV/PVA hydrogels during preparation process and the encapsulated cell density was controlled when the solutions were mixed. The change of the number of encapsulated cells and the storage modulus of the polymer/cell complex were measured through three days of incubation. The proliferation of the encapsulated cells was influenced by the storage modulus of the PMBV/PVA hydrogel. From this result, it was considered that the dynamical changes of the cells and their environment were evaluated through the physical properties of polymer/cell complex. The PMBV/PVA hydrogel is a promising platform for 3D cell engineering." @default.
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- W2005126370 date "2012-01-01" @default.
- W2005126370 modified "2023-10-05" @default.
- W2005126370 title "Cytocompatible polymer hydrogels as microenvironment tunable three-dimensional cell culture matrices" @default.
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- W2005126370 doi "https://doi.org/10.14723/tmrsj.37.357" @default.
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