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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005152425> ?p ?o ?g. }

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• W2005152425 abstract "Moth species have evolved integral membrane desaturases that exhibit a wide diversity in substrate specificity, as well as in regiospecificity and stereospecificity of the unsaturated products. We report here the cloning and expression of a single desaturase from the sex pheromone gland of the light brown apple moth, Epiphyas postvittana , that makes E 11 isomers of monounsaturated (E11-16 and E11-14) fatty acids and a diunsaturated (E9,E11-14) fatty acid. In the pheromone gland, the monoene precursor is made available by β oxidation of E11-16 acid with a subsequent two-carbon loss to E9-14 acid. A functional assay using a baculovirus expression system required addition of myristic acid and E9-14 acid precursors to demonstrate the unusual regiospecificity and stereospecificity of this desaturase. The amino acid sequence of this desaturase has ≈61% identity to that of Z 11-desaturases from two other insect species, and only ≈48% identity to the metabolic Z 9-desaturases in those species. A pheromone-gland Z 9-desaturase gene also was found with the light brown apple moth that differed in its deduced amino acid sequence (66% identity) with the metabolic Z 9-desaturase from fat body in this species." @default.
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• W2005152425 date "2002-01-22" @default.
• W2005152425 modified "2023-10-02" @default.
• W2005152425 title "Gene characterized for membrane desaturase that produces ( <i>E</i> )-11 isomers of mono- and diunsaturated fatty acids" @default.
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• W2005152425 doi "https://doi.org/10.1073/pnas.221601498" @default.
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