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- W2005156650 abstract "Adipose-derived stem cells (ASC) injected into the blood stream following an ischemic event promote therapeutic angiogenesis in affected tissues. It has been suggested that the stem cells exert their influence via a paracrine effect on native endothelial cells (EC). Using an in vitro model, we evaluate the effect of ASC co-culture on EC function in a hypoxic environment. Confluent monolayers of human EC grown on the bottom of transwell plates were wounded to create an even 5mm defect and cultured in either normoxic (21%) or hypoxic (1%) conditions. Human ASC were co-cultured on the top of the transwell plates to evaluate a paracrine effect. Subsequently, EC migration was determined by measuring the wound size after 3d. Media samples were collected and VEGF concentration measured via ELISA. To confirm mechanism, ECs were treated with recombinant VEGF at various concentrations and migration measured. HIF-1α expression was evaluated in ASC by Western blot. Hypoxia inhibited EC migration (0.87 mm vs 0.78mm, p < 0.05) over 3d. Co-culture of ASC enhanced EC migration in both normoxic (0.87 mm to 0.93mm) and hypoxic (0.78 mm to 1.02mm; p < 0.05) environments. Media from co-cultures in hypoxia contained significantly more VEGF (708.3 pg/mL) than normoxic co-cultures (311.2 pg/mL) and EC alone (28.9 pg/mL). The addition of VEGF to wounded EC cultures improved migration, but not to the extent of ASC co-culture. Finally, hypoxia increased levels of HIF-1α in ASC. These results demonstrate that: 1) ASC restore and enhance endothelial cell migratory function in a hypoxic environment, and 2) the effect is primarily, but not totally, due to secretion of VEGF by ASC in response to hypoxia. These results suggest that hypoxic pre-conditioning of ASC might be of value in enhancing their role in therapeutic angiogenesis to treat ischemic heart or limb conditions." @default.
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- W2005156650 date "2010-06-01" @default.
- W2005156650 modified "2023-09-30" @default.
- W2005156650 title "PS212. Human Adult Stem Cells Restore Endothelial Migratory Dysfunction in a Hypoxic Environment" @default.
- W2005156650 doi "https://doi.org/10.1016/j.jvs.2010.02.192" @default.
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