FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005165227> ?p ?o ?g. }

• W2005165227 endingPage "581" @default.
• W2005165227 startingPage "576" @default.
• W2005165227 abstract "The c-Jun N-terminal Kinases (JNKs) play important roles in cell responses to stress or growth factor stimulation. The JNK1α1 isoform shares >90% identity with a predominantly neuronal JNK3α1 isoform, but JNK3α1 also includes a distinctive 38 amino acid N-terminal sequence. To address the outstanding question of the potential for these JNK isoforms to have different binding partners that mediate different biological actions, the work presented here refined the yeast two-hybrid approach to identify and categorize binding partners for JNK1α1 and JNK3α1. Specifically, site-directed mutagenesis of the JNK1α1 common docking (CD) domain that mediates typical JNK-binding domain (JBD)-dependent interactions, truncation of the distinctive JNK3 N-terminal domain (i.e. ΔN JNK3α1), and interaction evaluation in the yeast two-hybrid system defined the interacting partners as either JNK1-specific interactors (ATF7, FUS, KCNE4, PIAS1, SHANK1, TKT), typical JBD-dependent interactors shared by JNK1α1 and JNK3α1 (AKAP6, BMPR2, EEF1A1, GFAP, GRIP2, GTF2F1, HDAC2, MAP1B, MYO9B, PTPN2, RABGAP1, RUSC2, SUMO1, SYPL1, TOPBP1, ZNF668), or JNK3-specific partners (ATXN1, NNAT, PTGDS) dependent on interaction with the JNK3 N-terminal extension. The interacting partners ATF7, AKAP6, and ATXN1 were explored further as representatives of these different classes. Two potential JBDs were identified in ATF7 as important for its interaction with JNK1α1, but additionally an interaction between ATF7 and ΔN JNK3α1 was shown to be JBD-dependent, suggesting that the JNK3α1 N-terminus prevents interaction with some proteins. For the shared partner AKAP6, one of the multiple potential JBDs predicted by sequence analysis was important for the AKAP6-JNK interaction in the yeast screening system as well as in mammalian cells. Finally, the ATXN1-JNK3α1 interaction was dependent on the JNK3α1 N-terminus in a mammalian cell context. These studies therefore highlight a diversity of potential JNK-interacting partners with both JBD-dependent as well as JBD-independent modes of interaction." @default.
• W2005165227 created "2016-06-24" @default.
• W2005165227 creator A5034128709 @default.
• W2005165227 creator A5045433710 @default.
• W2005165227 creator A5084648761 @default.
• W2005165227 date "2014-10-01" @default.
• W2005165227 modified "2023-10-18" @default.
• W2005165227 title "The JNK1/JNK3 interactome – Contributions by the JNK3 unique N-terminus and JNK common docking site residues" @default.
• W2005165227 cites W1530284699 @default.
• W2005165227 cites W1572174728 @default.
• W2005165227 cites W1972788136 @default.
• W2005165227 cites W1982499214 @default.
• W2005165227 cites W1984665850 @default.
• W2005165227 cites W1992068984 @default.
• W2005165227 cites W1993643447 @default.
• W2005165227 cites W2016008760 @default.
• W2005165227 cites W2044269995 @default.
• W2005165227 cites W2047563098 @default.
• W2005165227 cites W2047604959 @default.
• W2005165227 cites W2056570718 @default.
• W2005165227 cites W2061699263 @default.
• W2005165227 cites W2068251639 @default.
• W2005165227 cites W2083117403 @default.
• W2005165227 cites W2109709409 @default.
• W2005165227 cites W2114546262 @default.
• W2005165227 cites W2121438864 @default.
• W2005165227 cites W2129737293 @default.
• W2005165227 cites W2132108032 @default.
• W2005165227 cites W2137726406 @default.
• W2005165227 cites W2145965566 @default.
• W2005165227 cites W2149789552 @default.
• W2005165227 cites W2159178453 @default.
• W2005165227 cites W2160262717 @default.
• W2005165227 cites W2328985298 @default.
• W2005165227 doi "https://doi.org/10.1016/j.bbrc.2014.09.122" @default.
• W2005165227 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25301550" @default.
• W2005165227 hasPublicationYear "2014" @default.
• W2005165227 type Work @default.
• W2005165227 sameAs 2005165227 @default.
• W2005165227 citedByCount "9" @default.
• W2005165227 countsByYear W20051652272015 @default.
• W2005165227 countsByYear W20051652272016 @default.
• W2005165227 countsByYear W20051652272019 @default.
• W2005165227 countsByYear W20051652272020 @default.
• W2005165227 countsByYear W20051652272021 @default.
• W2005165227 countsByYear W20051652272022 @default.
• W2005165227 crossrefType "journal-article" @default.
• W2005165227 hasAuthorship W2005165227A5034128709 @default.
• W2005165227 hasAuthorship W2005165227A5045433710 @default.
• W2005165227 hasAuthorship W2005165227A5084648761 @default.
• W2005165227 hasConcept C104317684 @default.
• W2005165227 hasConcept C107824862 @default.
• W2005165227 hasConcept C159110408 @default.
• W2005165227 hasConcept C41685203 @default.
• W2005165227 hasConcept C515207424 @default.
• W2005165227 hasConcept C53345823 @default.
• W2005165227 hasConcept C54355233 @default.
• W2005165227 hasConcept C57711820 @default.
• W2005165227 hasConcept C71924100 @default.
• W2005165227 hasConcept C86803240 @default.
• W2005165227 hasConcept C9760119 @default.
• W2005165227 hasConceptScore W2005165227C104317684 @default.
• W2005165227 hasConceptScore W2005165227C107824862 @default.
• W2005165227 hasConceptScore W2005165227C159110408 @default.
• W2005165227 hasConceptScore W2005165227C41685203 @default.
• W2005165227 hasConceptScore W2005165227C515207424 @default.
• W2005165227 hasConceptScore W2005165227C53345823 @default.
• W2005165227 hasConceptScore W2005165227C54355233 @default.
• W2005165227 hasConceptScore W2005165227C57711820 @default.
• W2005165227 hasConceptScore W2005165227C71924100 @default.
• W2005165227 hasConceptScore W2005165227C86803240 @default.
• W2005165227 hasConceptScore W2005165227C9760119 @default.
• W2005165227 hasIssue "3" @default.
• W2005165227 hasLocation W20051652271 @default.
• W2005165227 hasLocation W20051652272 @default.
• W2005165227 hasOpenAccess W2005165227 @default.
• W2005165227 hasPrimaryLocation W20051652271 @default.
• W2005165227 hasRelatedWork W1953752353 @default.
• W2005165227 hasRelatedWork W1972413977 @default.
• W2005165227 hasRelatedWork W2613019168 @default.
• W2005165227 hasRelatedWork W2728300086 @default.
• W2005165227 hasRelatedWork W2749136581 @default.
• W2005165227 hasRelatedWork W2912479666 @default.
• W2005165227 hasRelatedWork W3148908243 @default.
• W2005165227 hasRelatedWork W4200304137 @default.
• W2005165227 hasRelatedWork W4214581935 @default.
• W2005165227 hasRelatedWork W4328053009 @default.
• W2005165227 hasVolume "453" @default.
• W2005165227 isParatext "false" @default.
• W2005165227 isRetracted "false" @default.
• W2005165227 magId "2005165227" @default.
• W2005165227 workType "article" @default.