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- W2005167997 abstract "Abstract The effect of eye carotenoid content, light conditions and retinoid supply on the biosynthesis of opsin, as well as the ability to isomerize of exogenous all- trans -retinal to 11- cis -retinal were investigated in the photoreceptors of blowfly Calliphora . SDS-PAGE of digitonin extracts from isolated rhabdom fractions of carotenoid-fortified and carotenoid-deficient animals revealed, on heavily loaded gels, that in both cases opsin forms faint minor bands similar to the patterns of “R − -flies” described as “vitamin A-deficient flies” (Paulsen and Schwemer, Biochim. biophys Acta 557 , 358–390, 1979) or “carotenoid-deficient flies” (Paulsen and Schwemer, Eur. J. Biochem. 137, 609–614, 1983 . Similar opsin patterns were obtained in flies subjected to continuous 72 h blue or yellow-green light or to a 12 h: 12 h white light:dark cycle, or total darkness, irrespective of the carotenoid content in their eyes. 11- cis -retinal and, to a lesser extent, all- trans -retinal, when included in the diet or painted on the cornea, were found to stimulate opsin biosynthesis in the dark. 11- cis -retinal in the dark or all- trans -retinal after illumination of the flies with blue light were the most effective, as compared with the effect of all- trans -retinal in the dark. Exogenous all- trans -retinal in the dark can be partially converted into a mixture of 11- cis -retinal (26%) and 13- cis -retinal (∼74%) by fly retina homogenate in vitro . It was concluded that Calliphora opsin biosynthesis is not strongly dependent on the carotenoid supply or on light: dark conditions and is triggered by the 11- cis aldehyde form of the chromophore, which can be produced in the fly retina either by an isomerase system in the dark or as a result of photoisomerization." @default.
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- W2005167997 title "Opsin biosynthesis and trans-cis isomerization of aldehyde form chromophore in the blowfly Calliphora erythrocephala eye" @default.
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- W2005167997 doi "https://doi.org/10.1016/0965-1748(92)90119-y" @default.
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