Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005172972> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2005172972 endingPage "467" @default.
- W2005172972 startingPage "459" @default.
- W2005172972 abstract "Two assays have been devised to demonstrate ATP-dependent migration of 40S ribosomal subunits on messenger RNA. The first is a two-step runoff assay. Reovirus mRNA was initially loaded with 40S subunits by incubation with wheat germ ribosomes in the presence of the antibiotic edeine. During the second phase of the incubation, in which further attachment of ribosomes was inhibited, the preformed complexes were shown to dissociate (presumably by runoff) only if ATP was included in the reaction. A more direct demonstration of ATP-dependent migration of 40S subunits was carried out using 3′ end-labeled brome mosaic virus mRNA. In the presence of edeine and ATP, 40S ribosomal subunits were shown to advance all the way to the 3′ end of the message, as shown by protection of the labeled 3′-proximal segment against nuclease digestion. Depletion of ATP by the addition of hexokinase prevented this migration. A variety of observations has raised the possibility that attachment of eucaryotic ribosomes to messenger RNA proceeds via a “scanning mechanism.” The hypothesis is that a 40S subunit binds initially at or near the 5′ terminus of the message and subsequently migrates toward the interior, stopping when it encounters the first AUG triplet. If migration of 40S subunits requires ATP, as the present studies suggest, the scanning mechanism predicts that in a system depleted of ATP a single 40S ribosome should be trapped near the 5′ terminus of the message—upstream of the AUG initiator codon. This prediction was confirmed by analyzing binding of wheat germ ribosomes to a synthetic ribopolymer in which the 5′-proximal region (lacking AUG codons) and the AUG-containing segment near the 3′ end of the molecule were differentially labeled." @default.
- W2005172972 created "2016-06-24" @default.
- W2005172972 creator A5023781524 @default.
- W2005172972 date "1980-11-01" @default.
- W2005172972 modified "2023-09-25" @default.
- W2005172972 title "Role of ATP in binding and migration of 40S ribosomal subunits" @default.
- W2005172972 cites W1481908209 @default.
- W2005172972 cites W1528312245 @default.
- W2005172972 cites W1586695792 @default.
- W2005172972 cites W1599372957 @default.
- W2005172972 cites W1654622166 @default.
- W2005172972 cites W1659735400 @default.
- W2005172972 cites W1967663354 @default.
- W2005172972 cites W2010432686 @default.
- W2005172972 cites W2017662104 @default.
- W2005172972 cites W2035590329 @default.
- W2005172972 cites W2058649879 @default.
- W2005172972 cites W2066705273 @default.
- W2005172972 cites W2083070222 @default.
- W2005172972 cites W2397769746 @default.
- W2005172972 cites W60878782 @default.
- W2005172972 doi "https://doi.org/10.1016/0092-8674(80)90356-6" @default.
- W2005172972 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7448869" @default.
- W2005172972 hasPublicationYear "1980" @default.
- W2005172972 type Work @default.
- W2005172972 sameAs 2005172972 @default.
- W2005172972 citedByCount "140" @default.
- W2005172972 countsByYear W20051729722012 @default.
- W2005172972 countsByYear W20051729722013 @default.
- W2005172972 countsByYear W20051729722014 @default.
- W2005172972 countsByYear W20051729722015 @default.
- W2005172972 countsByYear W20051729722016 @default.
- W2005172972 countsByYear W20051729722017 @default.
- W2005172972 countsByYear W20051729722018 @default.
- W2005172972 countsByYear W20051729722019 @default.
- W2005172972 countsByYear W20051729722020 @default.
- W2005172972 crossrefType "journal-article" @default.
- W2005172972 hasAuthorship W2005172972A5023781524 @default.
- W2005172972 hasConcept C104317684 @default.
- W2005172972 hasConcept C105580179 @default.
- W2005172972 hasConcept C12554922 @default.
- W2005172972 hasConcept C129194359 @default.
- W2005172972 hasConcept C153911025 @default.
- W2005172972 hasConcept C2779535198 @default.
- W2005172972 hasConcept C301363 @default.
- W2005172972 hasConcept C41258723 @default.
- W2005172972 hasConcept C55493867 @default.
- W2005172972 hasConcept C67705224 @default.
- W2005172972 hasConcept C67905577 @default.
- W2005172972 hasConcept C86803240 @default.
- W2005172972 hasConcept C88478588 @default.
- W2005172972 hasConcept C95444343 @default.
- W2005172972 hasConceptScore W2005172972C104317684 @default.
- W2005172972 hasConceptScore W2005172972C105580179 @default.
- W2005172972 hasConceptScore W2005172972C12554922 @default.
- W2005172972 hasConceptScore W2005172972C129194359 @default.
- W2005172972 hasConceptScore W2005172972C153911025 @default.
- W2005172972 hasConceptScore W2005172972C2779535198 @default.
- W2005172972 hasConceptScore W2005172972C301363 @default.
- W2005172972 hasConceptScore W2005172972C41258723 @default.
- W2005172972 hasConceptScore W2005172972C55493867 @default.
- W2005172972 hasConceptScore W2005172972C67705224 @default.
- W2005172972 hasConceptScore W2005172972C67905577 @default.
- W2005172972 hasConceptScore W2005172972C86803240 @default.
- W2005172972 hasConceptScore W2005172972C88478588 @default.
- W2005172972 hasConceptScore W2005172972C95444343 @default.
- W2005172972 hasIssue "2" @default.
- W2005172972 hasLocation W20051729721 @default.
- W2005172972 hasLocation W20051729722 @default.
- W2005172972 hasOpenAccess W2005172972 @default.
- W2005172972 hasPrimaryLocation W20051729721 @default.
- W2005172972 hasRelatedWork W133826215 @default.
- W2005172972 hasRelatedWork W17279961 @default.
- W2005172972 hasRelatedWork W2008993245 @default.
- W2005172972 hasRelatedWork W2030965575 @default.
- W2005172972 hasRelatedWork W2116360991 @default.
- W2005172972 hasRelatedWork W2217764468 @default.
- W2005172972 hasRelatedWork W2256924391 @default.
- W2005172972 hasRelatedWork W2408400528 @default.
- W2005172972 hasRelatedWork W2517092495 @default.
- W2005172972 hasRelatedWork W2570759695 @default.
- W2005172972 hasVolume "22" @default.
- W2005172972 isParatext "false" @default.
- W2005172972 isRetracted "false" @default.
- W2005172972 magId "2005172972" @default.
- W2005172972 workType "article" @default.