FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005182431> ?p ?o ?g. }

• W2005182431 endingPage "10" @default.
• W2005182431 startingPage "1" @default.
• W2005182431 abstract "Neuronal adhesion plays a fundamental role in growth, migration, regeneration and plasticity of neurons. However, current methods for studying neuronal adhesion cannot monitor this phenomenon quantitatively in real-time. In this work, we demonstrate the use of an acoustic sensor to measure adhesion of neuro-blastoma cells (Neuro-2A) in real-time. An acoustic sensor consisting of a quartz crystal sandwiched between gold electrodes was placed in a flow cell and filled with 600 μl of phosphate buffered saline (PBS). Two sets of in vitro experiments were performed using sensors that had uncoated gold electrodes and sensors that were coated with a known neuronal adhesion promoter (poly-l-lysine or PLL). The instantaneous resonant frequency and the equivalent motional resistance of the acoustic sensor were monitored every second. Cell Tracker™ was used to confirm neuronal adhesion to the surface. Addition of 10 μl of media and Neuro-2A cells into the above set-up elicited exponential changes in the resonant frequency and motional resistance of the quartz crystal with time to reach steady state in the range of 2–11 h. The steady-state change in resonant frequency in response to addition of neurons was linearly related to the number of Neuro-2A cells added (R2 = 0.94). Acoustic sensors coated with the adhesion promoter, PLL showed a much higher change in resonant frequency for approximately the same number of neurons. We conclude that the acoustic sensor has sufficient sensitivity to monitor neuronal adhesion in real-time. This has potential applications in the study of mechanisms of neuron–substrate interactions and the effect of molecular modulators in the extra cellular matrix." @default.
• W2005182431 created "2016-06-24" @default.
• W2005182431 creator A5015117536 @default.
• W2005182431 creator A5070590528 @default.
• W2005182431 creator A5072427810 @default.
• W2005182431 date "2005-05-01" @default.
• W2005182431 modified "2023-09-24" @default.
• W2005182431 title "Acoustic sensor for monitoring adhesion of Neuro-2A cells in real-time" @default.
• W2005182431 cites W1596248898 @default.
• W2005182431 cites W1964639134 @default.
• W2005182431 cites W1965381196 @default.
• W2005182431 cites W1970847048 @default.
• W2005182431 cites W1972537416 @default.
• W2005182431 cites W1972703119 @default.
• W2005182431 cites W1975997726 @default.
• W2005182431 cites W1978280489 @default.
• W2005182431 cites W1979822041 @default.
• W2005182431 cites W1982454520 @default.
• W2005182431 cites W1985838524 @default.
• W2005182431 cites W1993126272 @default.
• W2005182431 cites W1995764503 @default.
• W2005182431 cites W1996474109 @default.
• W2005182431 cites W2004404788 @default.
• W2005182431 cites W2006501418 @default.
• W2005182431 cites W2011645131 @default.
• W2005182431 cites W2018371812 @default.
• W2005182431 cites W2020664256 @default.
• W2005182431 cites W2025076833 @default.
• W2005182431 cites W2033836235 @default.
• W2005182431 cites W2034216099 @default.
• W2005182431 cites W2039152606 @default.
• W2005182431 cites W2044569816 @default.
• W2005182431 cites W2044973375 @default.
• W2005182431 cites W2055439074 @default.
• W2005182431 cites W2067862351 @default.
• W2005182431 cites W2070727423 @default.
• W2005182431 cites W2075558783 @default.
• W2005182431 cites W2080358190 @default.
• W2005182431 cites W2080678112 @default.
• W2005182431 cites W2080697543 @default.
• W2005182431 cites W2081119443 @default.
• W2005182431 cites W2110339757 @default.
• W2005182431 cites W2124897445 @default.
• W2005182431 cites W2136388349 @default.
• W2005182431 cites W2150363330 @default.
• W2005182431 cites W2158175581 @default.
• W2005182431 cites W2158560940 @default.
• W2005182431 cites W2167800894 @default.
• W2005182431 cites W2170289870 @default.
• W2005182431 cites W2599199563 @default.
• W2005182431 doi "https://doi.org/10.1016/j.jneumeth.2004.09.029" @default.
• W2005182431 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15848233" @default.
• W2005182431 hasPublicationYear "2005" @default.
• W2005182431 type Work @default.
• W2005182431 sameAs 2005182431 @default.
• W2005182431 citedByCount "12" @default.
• W2005182431 countsByYear W20051824312012 @default.
• W2005182431 countsByYear W20051824312013 @default.
• W2005182431 countsByYear W20051824312015 @default.
• W2005182431 countsByYear W20051824312019 @default.
• W2005182431 crossrefType "journal-article" @default.
• W2005182431 hasAuthorship W2005182431A5015117536 @default.
• W2005182431 hasAuthorship W2005182431A5070590528 @default.
• W2005182431 hasAuthorship W2005182431A5072427810 @default.
• W2005182431 hasConcept C12554922 @default.
• W2005182431 hasConcept C136229726 @default.
• W2005182431 hasConcept C147789679 @default.
• W2005182431 hasConcept C159985019 @default.
• W2005182431 hasConcept C17525397 @default.
• W2005182431 hasConcept C185592680 @default.
• W2005182431 hasConcept C192562407 @default.
• W2005182431 hasConcept C71924100 @default.
• W2005182431 hasConcept C84416704 @default.
• W2005182431 hasConcept C86803240 @default.
• W2005182431 hasConceptScore W2005182431C12554922 @default.
• W2005182431 hasConceptScore W2005182431C136229726 @default.
• W2005182431 hasConceptScore W2005182431C147789679 @default.
• W2005182431 hasConceptScore W2005182431C159985019 @default.
• W2005182431 hasConceptScore W2005182431C17525397 @default.
• W2005182431 hasConceptScore W2005182431C185592680 @default.
• W2005182431 hasConceptScore W2005182431C192562407 @default.
• W2005182431 hasConceptScore W2005182431C71924100 @default.
• W2005182431 hasConceptScore W2005182431C84416704 @default.
• W2005182431 hasConceptScore W2005182431C86803240 @default.
• W2005182431 hasIssue "1" @default.
• W2005182431 hasLocation W20051824311 @default.
• W2005182431 hasLocation W20051824312 @default.
• W2005182431 hasOpenAccess W2005182431 @default.
• W2005182431 hasPrimaryLocation W20051824311 @default.
• W2005182431 hasRelatedWork W1507683687 @default.
• W2005182431 hasRelatedWork W2003830875 @default.
• W2005182431 hasRelatedWork W2167733270 @default.
• W2005182431 hasRelatedWork W2327242670 @default.
• W2005182431 hasRelatedWork W2399868924 @default.
• W2005182431 hasRelatedWork W2899084033 @default.
• W2005182431 hasRelatedWork W2902552153 @default.
• W2005182431 hasRelatedWork W3018773520 @default.
• W2005182431 hasRelatedWork W3038568766 @default.

• next