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- W2005191553 abstract "The ability to store platelets beyond 24 hours requires a functionally closed system. This study tested the ability of a cell separator bowl seal system to resist penetration of microbial contamination under normal running conditions and under extreme environmental stress. Three test organisms, Micrococcus luteus, Serratia marcescens, and Staphylococcus epidermidis, were applied directly to the bowl at the edge of the seal or aerosolized and passed through the centrifuge chamber while the cell separator was run through a simulated platelet collection. A sterile, bacteriologic nutrient medium was perfused through the tubing set, thus simulating the flow of blood fractions. Following the procedure, the medium was examined for microbial growth. The concentration of aerosolized bacteria ranged from 5.2 times 101 to 3.9 times 103 colony-forming units (CFU) per mL, and the concentration of bacteria applied to the edge of the seal ranged from 1.9 times 105 to 2.8 times 109 CFU per mL. The positive control, direct inoculation of S. marcescens into the circulating medium (50 CFU/500 mL), resulted in recovery of the identical organism after 24 hours' incubation. No contamination of the system was detected in 40 experiments with aerosolized bacteria or in 32 experiments in which bacteria were applied directly to the seal. This study demonstrates that this sealed-bowl system resists microbial contamination." @default.
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- W2005191553 date "1990-02-01" @default.
- W2005191553 modified "2023-09-24" @default.
- W2005191553 title "Microbial challenge of a blood cell separator outside-seal bowl system" @default.
- W2005191553 doi "https://doi.org/10.1046/j.1537-2995.1990.30290162901.x" @default.
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