FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005202466> ?p ?o ?g. }

• W2005202466 endingPage "e12991" @default.
• W2005202466 startingPage "e12991" @default.
• W2005202466 abstract "The differentiation of fibroblast-like pre-adipocytes to lipid-loaded adipocytes is regulated by a network of transcription factors, the most prominent one being the nuclear receptor peroxisome proliferator-activated receptor (PPAR) γ. However, many of the other 47 members of the nuclear receptor superfamily have an impact on adipogenesis, which in human cells has not been investigated in detail.We analyzed by quantitative PCR all human nuclear receptors at multiple time points during differentiation of SGBS pre-adipocytes. The earliest effect was the down-regulation of the genes RARG, PPARD, REV-ERBA, REV-ERBB, VDR and GR followed by the up-regulation of PPARG, LXRA and AR. These observations are supported with data from 3T3-L1 mouse pre-adipocytes and primary human adipocytes. Investigation of the effects of the individual differentiation mix components in short-term treatments and of their omission from the full mix showed that the expression levels of the early-regulated nuclear receptor genes were most affected by the glucocorticoid receptor (GR) ligand cortisol and the phosphodiesterase inhibitor IBMX. Interestingly, the effects of both compounds converged to repress the genes PPARD, REV-ERBA, REV-ERBB, VDR and GR, whereas cortisol and IBMX showed antagonistic interaction for PPARG, LXRA and AR causing a time lag in their up-regulation. We hypothesize that the well-known auto-repression of GR fine-tunes the detected early responses. Consistently, chromatin immunoprecipitation experiments showed that GR association increased on the transcription start sites of the genes RARG, REV-ERBB, VDR and GR.Adipocyte differentiation is a process, in which many members of the nuclear receptor superfamily change their mRNA expression. The actions of cortisol and IBMX converged to repress several nuclear receptors early in differentiation, while up-regulation of other nuclear receptor genes showed a time lag due to antagonisms of the signals. Our results place GR and its ligand cortisol as central regulatory factors controlling early regulatory events in human adipogenesis that precedes the regulation of the later events by PPARG." @default.
• W2005202466 created "2016-06-24" @default.
• W2005202466 creator A5035613777 @default.
• W2005202466 creator A5072393510 @default.
• W2005202466 creator A5074126248 @default.
• W2005202466 creator A5082171858 @default.
• W2005202466 creator A5089547723 @default.
• W2005202466 date "2010-09-27" @default.
• W2005202466 modified "2023-10-14" @default.
• W2005202466 title "Time-Resolved Expression Profiling of the Nuclear Receptor Superfamily in Human Adipogenesis" @default.
• W2005202466 cites W1553656745 @default.
• W2005202466 cites W1804608618 @default.
• W2005202466 cites W180493639 @default.
• W2005202466 cites W1979385177 @default.
• W2005202466 cites W2000037541 @default.
• W2005202466 cites W2004411749 @default.
• W2005202466 cites W2010142538 @default.
• W2005202466 cites W2020541883 @default.
• W2005202466 cites W2026930722 @default.
• W2005202466 cites W2027704011 @default.
• W2005202466 cites W2034089831 @default.
• W2005202466 cites W2036468147 @default.
• W2005202466 cites W2046612545 @default.
• W2005202466 cites W2052609811 @default.
• W2005202466 cites W2055106896 @default.
• W2005202466 cites W2057313230 @default.
• W2005202466 cites W2060364237 @default.
• W2005202466 cites W2061425021 @default.
• W2005202466 cites W2063713206 @default.
• W2005202466 cites W2071692398 @default.
• W2005202466 cites W2075496997 @default.
• W2005202466 cites W2077700473 @default.
• W2005202466 cites W2079080809 @default.
• W2005202466 cites W2087685430 @default.
• W2005202466 cites W2088986234 @default.
• W2005202466 cites W2091673549 @default.
• W2005202466 cites W2095853712 @default.
• W2005202466 cites W2101951807 @default.
• W2005202466 cites W2103759127 @default.
• W2005202466 cites W2106443624 @default.
• W2005202466 cites W2106607972 @default.
• W2005202466 cites W2106636803 @default.
• W2005202466 cites W2113112889 @default.
• W2005202466 cites W2115188607 @default.
• W2005202466 cites W2115459204 @default.
• W2005202466 cites W2116039237 @default.
• W2005202466 cites W2118397648 @default.
• W2005202466 cites W2122463654 @default.
• W2005202466 cites W2126602684 @default.
• W2005202466 cites W2128900719 @default.
• W2005202466 cites W2131820599 @default.
• W2005202466 cites W2137712228 @default.
• W2005202466 cites W2138376605 @default.
• W2005202466 cites W2140347764 @default.
• W2005202466 cites W2140727482 @default.
• W2005202466 cites W2142535078 @default.
• W2005202466 cites W2142693509 @default.
• W2005202466 cites W2145805471 @default.
• W2005202466 cites W2148217900 @default.
• W2005202466 cites W2154114022 @default.
• W2005202466 cites W2156281856 @default.
• W2005202466 cites W2165649406 @default.
• W2005202466 cites W2167008303 @default.
• W2005202466 cites W2167372953 @default.
• W2005202466 cites W2605614532 @default.
• W2005202466 doi "https://doi.org/10.1371/journal.pone.0012991" @default.
• W2005202466 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2946337" @default.
• W2005202466 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20885999" @default.
• W2005202466 hasPublicationYear "2010" @default.
• W2005202466 type Work @default.
• W2005202466 sameAs 2005202466 @default.
• W2005202466 citedByCount "27" @default.
• W2005202466 countsByYear W20052024662012 @default.
• W2005202466 countsByYear W20052024662013 @default.
• W2005202466 countsByYear W20052024662014 @default.
• W2005202466 countsByYear W20052024662015 @default.
• W2005202466 countsByYear W20052024662016 @default.
• W2005202466 countsByYear W20052024662018 @default.
• W2005202466 countsByYear W20052024662019 @default.
• W2005202466 countsByYear W20052024662020 @default.
• W2005202466 countsByYear W20052024662021 @default.
• W2005202466 countsByYear W20052024662023 @default.
• W2005202466 crossrefType "journal-article" @default.
• W2005202466 hasAuthorship W2005202466A5035613777 @default.
• W2005202466 hasAuthorship W2005202466A5072393510 @default.
• W2005202466 hasAuthorship W2005202466A5074126248 @default.
• W2005202466 hasAuthorship W2005202466A5082171858 @default.
• W2005202466 hasAuthorship W2005202466A5089547723 @default.
• W2005202466 hasBestOaLocation W20052024661 @default.
• W2005202466 hasConcept C101762097 @default.
• W2005202466 hasConcept C104317684 @default.
• W2005202466 hasConcept C122927707 @default.
• W2005202466 hasConcept C126322002 @default.
• W2005202466 hasConcept C130241916 @default.
• W2005202466 hasConcept C134018914 @default.
• W2005202466 hasConcept C134320426 @default.
• W2005202466 hasConcept C150194340 @default.
• W2005202466 hasConcept C170493617 @default.

• next