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• W2005213706 abstract "Serum-sensitive mutants and their serum-resistant smooth parental E. coli strains (Wf8, Wf26, and WF 52) have been investigated in respect to their binding of different complement components. These pairs consisting of a wild-type and its mutants represent a better model for the investigation of the mechanism of serum resistance than the comparison of unrelated strains. Both strains of a pair bind equivalent amounts of C3. In binding assays using radiolabeled terminal components C6, C7, C8, and C9, the serum-sensitive strains do bind more late acting components than their resistant parental strains. An active membrane attack complex stably bound to the cell surface was found on the mutants, whereas with wild-type bacteria a complex could be isolated from the supernatant which is composed of the late acting complement components and S-protein. This complex is released from the surface of the wild-type bacteria without participation of C9. Serum-sensitive Mutanten und ihre serum-resistenten glatten Ausgangsstämme (E. coli Wf8, Wf28 and Wf52) wurden im Hinblick auf ihre Bindung bzw. Aktivierung von Komplementkomponenten untersucht. Diese Bakterienpaare aus serum-resistentem Wildtyp und serum-sensiblen Mutanten sind ein besseres Modell, um den Mechanismus der Serumresistenz zu untersuchen als der Vergleich von unterschiedlichen Stämmen. Die jeweiligen Paare banden gleiche Mengen von C3. Bei Bindungsstudien mit radioaktiv markiertem C6, C7, C8 und C9 wurde gefunden, daß die serum-sensiblen Stämme mehr „late-acting“-Komponenten binden als ihre serum-resistenten Elternstämme. Ein kompletter C5b-9-Komplex konnte aus den Membranen der serum-sensiblen Mutanten nachgewiesen werden. Bei den Wildtyp-Ausgangsstämmen wurde hingegen im Überstand ein Makromolekülkomplex gefunden, der aus C5b, C6, C7, C8 und dem S-Protein bestand. Dieser Komplex dissoziiert offenbar von der Oberfläche der Wildtypen ab, ohne daß C9 daran beteiligt ist." @default.
• W2005213706 created "2016-06-24" @default.
• W2005213706 creator A5000300308 @default.
• W2005213706 creator A5088762139 @default.
• W2005213706 date "1988-11-01" @default.
• W2005213706 modified "2023-09-26" @default.
• W2005213706 title "Studies on Serum Resistance in E scherichia coli" @default.
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• W2005213706 doi "https://doi.org/10.1016/s0176-6724(88)80141-x" @default.
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