FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005220250> ?p ?o ?g. }

• W2005220250 endingPage "231" @default.
• W2005220250 startingPage "221" @default.
• W2005220250 abstract "Recombinant amidase from Sulfolobus solfataricus occurred as a dimer of 110 kDa comprising identical subunits. Only dimers were present at pHs above 7.0, but with decreasing pH, dimers associated into octamers, with complete oligomerization occurring at pH 3.0. Oligomerization showed reversible temperature-dependence, with octamer formation increasing with temperature from 36 °C to between 70 and 80° C. Increasing salt concentrations, favored dissociation of the octamers. Among the three investigated factors affecting the dimer–octamer equilibrium, the most important was pH. Among four mutants obtained by site-specific mutagenesis and selection for pH and temperature sensitivity, the T319I and D487N mutant amidases, like that of the native Sulfolobus solfataricus , responded to changes in pH and temperature with a conformational change affecting the dimer–octamer equilibrium. The Y41C and L34P mutant amidases were unaffected by pH and temperature, remaining always in the dimeric state. The differences among mutants in protein conformation must be related to the position of the introduced mutation. Although the L34P and Y41C mutations are located in the helical region 33–48 (LLKLQLESYERLDSLP), which is close to the amino-terminal segment of the protein, the T319I mutation is located in a strand on the surface of the protein, which is far from, and opposite to, the amino-terminal segment. The D487N mutation is located in the center of the protein, far distant from the 33–48 segment. These observations suggest that the segment of the protein closest to the amino-terminus plays a key role in the association of dimers into octamers." @default.
• W2005220250 created "2016-06-24" @default.
• W2005220250 creator A5010174099 @default.
• W2005220250 creator A5038393114 @default.
• W2005220250 creator A5053627554 @default.
• W2005220250 creator A5061191212 @default.
• W2005220250 creator A5074286800 @default.
• W2005220250 creator A5080693925 @default.
• W2005220250 creator A5091141052 @default.
• W2005220250 date "2009-01-01" @default.
• W2005220250 modified "2023-10-12" @default.
• W2005220250 title "pH-, temperature- and ion-dependent oligomerization ofSulfolobus solfataricusrecombinant amidase: a study with site-specific mutants" @default.
• W2005220250 cites W1483099488 @default.
• W2005220250 cites W1518219009 @default.
• W2005220250 cites W1636945110 @default.
• W2005220250 cites W1968212477 @default.
• W2005220250 cites W1969338731 @default.
• W2005220250 cites W1970191667 @default.
• W2005220250 cites W1973363547 @default.
• W2005220250 cites W1979402355 @default.
• W2005220250 cites W1984460745 @default.
• W2005220250 cites W1995523236 @default.
• W2005220250 cites W2000713499 @default.
• W2005220250 cites W2005986251 @default.
• W2005220250 cites W2006975595 @default.
• W2005220250 cites W2012720430 @default.
• W2005220250 cites W2015623521 @default.
• W2005220250 cites W2027991357 @default.
• W2005220250 cites W2081804305 @default.
• W2005220250 cites W2092621707 @default.
• W2005220250 cites W2099752571 @default.
• W2005220250 cites W2133792795 @default.
• W2005220250 cites W2147302373 @default.
• W2005220250 cites W2157400187 @default.
• W2005220250 doi "https://doi.org/10.1155/2009/280317" @default.
• W2005220250 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2686392" @default.
• W2005220250 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19478917" @default.
• W2005220250 hasPublicationYear "2009" @default.
• W2005220250 type Work @default.
• W2005220250 sameAs 2005220250 @default.
• W2005220250 citedByCount "10" @default.
• W2005220250 countsByYear W20052202502012 @default.
• W2005220250 countsByYear W20052202502013 @default.
• W2005220250 countsByYear W20052202502017 @default.
• W2005220250 countsByYear W20052202502018 @default.
• W2005220250 countsByYear W20052202502019 @default.
• W2005220250 countsByYear W20052202502021 @default.
• W2005220250 crossrefType "journal-article" @default.
• W2005220250 hasAuthorship W2005220250A5010174099 @default.
• W2005220250 hasAuthorship W2005220250A5038393114 @default.
• W2005220250 hasAuthorship W2005220250A5053627554 @default.
• W2005220250 hasAuthorship W2005220250A5061191212 @default.
• W2005220250 hasAuthorship W2005220250A5074286800 @default.
• W2005220250 hasAuthorship W2005220250A5080693925 @default.
• W2005220250 hasAuthorship W2005220250A5091141052 @default.
• W2005220250 hasBestOaLocation W20052202501 @default.
• W2005220250 hasConcept C104317684 @default.
• W2005220250 hasConcept C143065580 @default.
• W2005220250 hasConcept C16318435 @default.
• W2005220250 hasConcept C173681060 @default.
• W2005220250 hasConcept C178790620 @default.
• W2005220250 hasConcept C181199279 @default.
• W2005220250 hasConcept C185592680 @default.
• W2005220250 hasConcept C2777339483 @default.
• W2005220250 hasConcept C2779546866 @default.
• W2005220250 hasConcept C2780631447 @default.
• W2005220250 hasConcept C550995028 @default.
• W2005220250 hasConcept C55493867 @default.
• W2005220250 hasConcept C64927066 @default.
• W2005220250 hasConcept C65588867 @default.
• W2005220250 hasConcept C71240020 @default.
• W2005220250 hasConcept C84772758 @default.
• W2005220250 hasConceptScore W2005220250C104317684 @default.
• W2005220250 hasConceptScore W2005220250C143065580 @default.
• W2005220250 hasConceptScore W2005220250C16318435 @default.
• W2005220250 hasConceptScore W2005220250C173681060 @default.
• W2005220250 hasConceptScore W2005220250C178790620 @default.
• W2005220250 hasConceptScore W2005220250C181199279 @default.
• W2005220250 hasConceptScore W2005220250C185592680 @default.
• W2005220250 hasConceptScore W2005220250C2777339483 @default.
• W2005220250 hasConceptScore W2005220250C2779546866 @default.
• W2005220250 hasConceptScore W2005220250C2780631447 @default.
• W2005220250 hasConceptScore W2005220250C550995028 @default.
• W2005220250 hasConceptScore W2005220250C55493867 @default.
• W2005220250 hasConceptScore W2005220250C64927066 @default.
• W2005220250 hasConceptScore W2005220250C65588867 @default.
• W2005220250 hasConceptScore W2005220250C71240020 @default.
• W2005220250 hasConceptScore W2005220250C84772758 @default.
• W2005220250 hasFunder F4320321873 @default.
• W2005220250 hasIssue "4" @default.
• W2005220250 hasLocation W20052202501 @default.
• W2005220250 hasLocation W20052202502 @default.
• W2005220250 hasLocation W20052202503 @default.
• W2005220250 hasLocation W20052202504 @default.
• W2005220250 hasOpenAccess W2005220250 @default.
• W2005220250 hasPrimaryLocation W20052202501 @default.
• W2005220250 hasRelatedWork W1572025105 @default.
• W2005220250 hasRelatedWork W1894529473 @default.

• next