Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005255228> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2005255228 endingPage "793" @default.
- W2005255228 startingPage "788" @default.
- W2005255228 abstract "본 연구는 어류 병원균의 비 유전성을 가지는 항생제 내성균인 persister cell에 관한 연구이다. Persister cell은 기존의 항생제를 분해하는 저항균체(resistant cell)와는 다른 특성으로 항생제에 대한 저항성을 가지는데, 항생제가 존재하는 환경에서 새로운 기작으로 항생제에 대한 내성을 형성한다. 그래서 기존의 양식장에서 어류를 키울 때 어류에 투여하는 항생제는 일반적인 균주의 사멸 항생제 농도보다 더 높은 농도의 항생제를 투여하게 된다. 특히, E. tarda에 대한 다양한 항생제가 개발되어 있지만 내성균의 출현으로 그 효과가 좋지 않으며, 또한 persister cell에 의한 질병 재발을 방지하기 위해 균사멸 농도보다 훨씬 높은 농도의 항생제를 처리하고 있다. Persiser cell의 특이적인 저감 효과를 확인 하기 위하여 선별된 3종의 식물 추출물(돌외, 예덕나무, 상산)을 항생제와 함께 사용하였으며, 돌외와 예덕나무가 100 <TEX>${mu}g/ml$</TEX>, 상산은 200 <TEX>${mu}g/ml$</TEX>의 농도에서 persister cell의 사멸 효과를 나타내었다. 또한 넙치를 이용한 12일간의 누적 폐사율을 조사한 결과, 식물 추출물과 항생제 혼합액의 복강 투여구가 항생제 단독의 복강 투여구 보다 낮은 누적 폐사율이 관찰되었고, 항생제에 첨가한 식물 추출물의 농도가 돌외 30 <TEX>${mu}g/ml$</TEX>, 예덕나무 10 <TEX>${mu}g/ml$</TEX>, 상산 10 <TEX>${mu}g/ml$</TEX>의 농도 투여구에서 가장 낮은 누적 폐사율을 나타내었다. 따라서 본 연구에 사용된 세가지 추출물들(돌외, 예덕나무, 상산)은 항균 활성을 가지지 않은 농도에서 항생제와 병용하여 persister cell의 저감 효과가 있음을 확인하였다. High concentration of antibiotics has been used to treat the outbreak of edwardsiellosis caused by Edwardsiella tarda in aquaculture. However, not all of the bacteria have been killed with high concentrations of antibiotics treatment by the formation of persister cells with a dormant state. The main objective of this study was to kill persister cell using antibiotics with the addition of natural plant compounds. Antibiotics used in this study consist of 100 mg/ml florfenicol and 100 mg/ml amoxicillin. Ten natural plant compounds with persister cell inhibitor activity to E. coli were obtained from Protein Engineering and Systems Biology Lab. of Sungkyunkwan University. The persister cell inhibition activities of those natural plant compounds were evaluated in test tube. Concentrations of the antibiotics were in the ranges of 25~200 <TEX>${mu}g/ml$</TEX>. The persister cell formation was observed after 16 hours of culture. Persister cells were killed by antibiotics with natural plant compounds. Among ten natural plant compounds, Gynostemma pentaphyllum, Mallotus japonicus, and Orixa japonica showed persister cell formation inhibition activities. The optimal concentrations of G. pentaphyllum, M. japonicus, and O. japonica for the inhibitor of persister cell formation were 100 <TEX>${mu}g/ml$</TEX>, 100 <TEX>${mu}g/ml$</TEX>, and 200 <TEX>${mu}g/ml$</TEX>, respectively. In vivo study was carried out to evaluate the effect of the antibiotics with natural plant compounds using aquacultural fish, olive flounder, as test animals. G. pentaphyllum, M. japonicus, and O. japonica of 30 <TEX>${mu}g/ml$</TEX>, 10 <TEX>${mu}g/ml$</TEX>, and 10 <TEX>${mu}g/ml$</TEX> with antibiotics reduced cumulative mortalities, showing the effectiveness of persister cell inhibition." @default.
- W2005255228 created "2016-06-24" @default.
- W2005255228 creator A5007268142 @default.
- W2005255228 creator A5046827465 @default.
- W2005255228 creator A5076532152 @default.
- W2005255228 date "2012-06-30" @default.
- W2005255228 modified "2023-09-27" @default.
- W2005255228 title "Effects of Natural Compounds from Various Plant Eradicate the Persister Cell of Edwardsiella tarda Treated with Antibiotics of Florfenicol and Amoxicillin" @default.
- W2005255228 cites W1969244937 @default.
- W2005255228 cites W1992572387 @default.
- W2005255228 cites W2017165733 @default.
- W2005255228 cites W2045825103 @default.
- W2005255228 cites W2078564032 @default.
- W2005255228 cites W2115514927 @default.
- W2005255228 cites W2138412094 @default.
- W2005255228 cites W2145676608 @default.
- W2005255228 cites W2161021025 @default.
- W2005255228 cites W2323845517 @default.
- W2005255228 cites W2333313282 @default.
- W2005255228 doi "https://doi.org/10.5352/jls.2012.22.6.788" @default.
- W2005255228 hasPublicationYear "2012" @default.
- W2005255228 type Work @default.
- W2005255228 sameAs 2005255228 @default.
- W2005255228 citedByCount "1" @default.
- W2005255228 countsByYear W20052552282023 @default.
- W2005255228 crossrefType "journal-article" @default.
- W2005255228 hasAuthorship W2005255228A5007268142 @default.
- W2005255228 hasAuthorship W2005255228A5046827465 @default.
- W2005255228 hasAuthorship W2005255228A5076532152 @default.
- W2005255228 hasBestOaLocation W20052552281 @default.
- W2005255228 hasConcept C136622501 @default.
- W2005255228 hasConcept C2776832872 @default.
- W2005255228 hasConcept C2780663544 @default.
- W2005255228 hasConcept C501593827 @default.
- W2005255228 hasConcept C523546767 @default.
- W2005255228 hasConcept C54355233 @default.
- W2005255228 hasConcept C58123911 @default.
- W2005255228 hasConcept C86803240 @default.
- W2005255228 hasConcept C89423630 @default.
- W2005255228 hasConceptScore W2005255228C136622501 @default.
- W2005255228 hasConceptScore W2005255228C2776832872 @default.
- W2005255228 hasConceptScore W2005255228C2780663544 @default.
- W2005255228 hasConceptScore W2005255228C501593827 @default.
- W2005255228 hasConceptScore W2005255228C523546767 @default.
- W2005255228 hasConceptScore W2005255228C54355233 @default.
- W2005255228 hasConceptScore W2005255228C58123911 @default.
- W2005255228 hasConceptScore W2005255228C86803240 @default.
- W2005255228 hasConceptScore W2005255228C89423630 @default.
- W2005255228 hasIssue "6" @default.
- W2005255228 hasLocation W20052552281 @default.
- W2005255228 hasOpenAccess W2005255228 @default.
- W2005255228 hasPrimaryLocation W20052552281 @default.
- W2005255228 hasRelatedWork W1968602861 @default.
- W2005255228 hasRelatedWork W2011822037 @default.
- W2005255228 hasRelatedWork W2042669010 @default.
- W2005255228 hasRelatedWork W2406107337 @default.
- W2005255228 hasRelatedWork W2591998335 @default.
- W2005255228 hasRelatedWork W2616430012 @default.
- W2005255228 hasRelatedWork W2801332876 @default.
- W2005255228 hasRelatedWork W2808084198 @default.
- W2005255228 hasRelatedWork W2810544809 @default.
- W2005255228 hasRelatedWork W3429368 @default.
- W2005255228 hasVolume "22" @default.
- W2005255228 isParatext "false" @default.
- W2005255228 isRetracted "false" @default.
- W2005255228 magId "2005255228" @default.
- W2005255228 workType "article" @default.