Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005264045> ?p ?o ?g. }
- W2005264045 endingPage "346" @default.
- W2005264045 startingPage "335" @default.
- W2005264045 abstract "Hypothalamic AMP‐activated kinase (AMPK) is a key regulator of food intake in mammals. Its role in reproduction at the central level and, more precisely, in gonadotrophin‐releasing hormone (GnRH) release has never been investigated. We showed that each subunit of AMPK is present in immortalised GnRH neurones (GT1‐7 cells). Treatment with 5‐aminoimidazole‐4‐carboxamide‐1‐β‐ d ‐ribonucleoside (AICAR) and metformin, two activators of AMPK, increased dose‐dependent and time‐dependent phosphorylation of AMPKα atThr172 in GT1‐7 cells. Phosphorylation of acetyl‐coenzyme A carboxylase at ser79 also increased. Treatment with AICAR (5 m m ) or metformin (5 m m ) for 4 h inhibited GnRH release in the presence or absence of GnRH (10 −8 m ). Specific AMPK inhibitor compound C completely eliminated the effects of AICAR or metformin on GnRH release. Finally, we determined the central effects of AICAR in vivo on food intake and oestrous cyclicity. Ten‐week‐old female rats received a 50 μg AICAR or a saline i.c.v. injection. We detected increased AMPK and acetyl‐CoA carboxylase phosphorylation, specifically in the hypothalamus, 30 min after AICAR injection. Food intake was significantly higher (P < 0.05) in animals treated with AICAR than in animals injected with saline, 24 h after injection. This effect was abolished after 1 week. Moreover, during the 4 weeks following injection, the interval between two oestrous stages was significantly lower in the AICAR group than in the saline group. Our findings suggest that AMPK activation may act directly at the hypothalamic level to affect fertility by modulating GnRH release and oestrous cyclicity." @default.
- W2005264045 created "2016-06-24" @default.
- W2005264045 creator A5010534449 @default.
- W2005264045 creator A5015545254 @default.
- W2005264045 creator A5020421797 @default.
- W2005264045 creator A5022851366 @default.
- W2005264045 creator A5055542572 @default.
- W2005264045 creator A5055789689 @default.
- W2005264045 creator A5065146893 @default.
- W2005264045 creator A5081540288 @default.
- W2005264045 creator A5090496865 @default.
- W2005264045 creator A5091539631 @default.
- W2005264045 date "2008-01-11" @default.
- W2005264045 modified "2023-10-17" @default.
- W2005264045 title "The Effect of AMP‐Activated Kinase Activation on Gonadotrophin‐Releasing Hormone Secretion in GT1‐7 Cells and its Potential Role in Hypothalamic Regulation of the Oestrous Cyclicity in Rats" @default.
- W2005264045 cites W1919521423 @default.
- W2005264045 cites W1964881942 @default.
- W2005264045 cites W1964913810 @default.
- W2005264045 cites W1966491270 @default.
- W2005264045 cites W1971640401 @default.
- W2005264045 cites W1972762474 @default.
- W2005264045 cites W1973300372 @default.
- W2005264045 cites W1974118452 @default.
- W2005264045 cites W1979704616 @default.
- W2005264045 cites W1980316562 @default.
- W2005264045 cites W1983890824 @default.
- W2005264045 cites W1992243003 @default.
- W2005264045 cites W1994554353 @default.
- W2005264045 cites W1995402593 @default.
- W2005264045 cites W1996467928 @default.
- W2005264045 cites W2003580023 @default.
- W2005264045 cites W2016986133 @default.
- W2005264045 cites W2018739526 @default.
- W2005264045 cites W2022975427 @default.
- W2005264045 cites W2026300863 @default.
- W2005264045 cites W2029875505 @default.
- W2005264045 cites W2033823674 @default.
- W2005264045 cites W2035703541 @default.
- W2005264045 cites W2035905369 @default.
- W2005264045 cites W2042444876 @default.
- W2005264045 cites W2042706232 @default.
- W2005264045 cites W2045625894 @default.
- W2005264045 cites W2061026736 @default.
- W2005264045 cites W2065705538 @default.
- W2005264045 cites W2074303577 @default.
- W2005264045 cites W2079311820 @default.
- W2005264045 cites W2081304226 @default.
- W2005264045 cites W2095852400 @default.
- W2005264045 cites W2096555371 @default.
- W2005264045 cites W2096591765 @default.
- W2005264045 cites W2101351673 @default.
- W2005264045 cites W2107680033 @default.
- W2005264045 cites W2114952735 @default.
- W2005264045 cites W2117833492 @default.
- W2005264045 cites W2124536446 @default.
- W2005264045 cites W2128485668 @default.
- W2005264045 cites W2130769261 @default.
- W2005264045 cites W2133184757 @default.
- W2005264045 cites W2138226825 @default.
- W2005264045 cites W2143829406 @default.
- W2005264045 cites W2148606577 @default.
- W2005264045 cites W2154915084 @default.
- W2005264045 cites W2159536456 @default.
- W2005264045 cites W2164125044 @default.
- W2005264045 cites W2189514714 @default.
- W2005264045 cites W4206616952 @default.
- W2005264045 cites W4235273516 @default.
- W2005264045 cites W4247545511 @default.
- W2005264045 cites W4248245324 @default.
- W2005264045 cites W4250472606 @default.
- W2005264045 cites W1996556957 @default.
- W2005264045 doi "https://doi.org/10.1111/j.1365-2826.2007.01643.x" @default.
- W2005264045 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18194429" @default.
- W2005264045 hasPublicationYear "2008" @default.
- W2005264045 type Work @default.
- W2005264045 sameAs 2005264045 @default.
- W2005264045 citedByCount "59" @default.
- W2005264045 countsByYear W20052640452012 @default.
- W2005264045 countsByYear W20052640452013 @default.
- W2005264045 countsByYear W20052640452014 @default.
- W2005264045 countsByYear W20052640452015 @default.
- W2005264045 countsByYear W20052640452016 @default.
- W2005264045 countsByYear W20052640452017 @default.
- W2005264045 countsByYear W20052640452018 @default.
- W2005264045 countsByYear W20052640452019 @default.
- W2005264045 countsByYear W20052640452020 @default.
- W2005264045 countsByYear W20052640452021 @default.
- W2005264045 countsByYear W20052640452022 @default.
- W2005264045 countsByYear W20052640452023 @default.
- W2005264045 crossrefType "journal-article" @default.
- W2005264045 hasAuthorship W2005264045A5010534449 @default.
- W2005264045 hasAuthorship W2005264045A5015545254 @default.
- W2005264045 hasAuthorship W2005264045A5020421797 @default.
- W2005264045 hasAuthorship W2005264045A5022851366 @default.
- W2005264045 hasAuthorship W2005264045A5055542572 @default.
- W2005264045 hasAuthorship W2005264045A5055789689 @default.
- W2005264045 hasAuthorship W2005264045A5065146893 @default.
- W2005264045 hasAuthorship W2005264045A5081540288 @default.