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- W2005277635 abstract "The introduction of genetic variation from wild and cultivated Triticeae species has been a long-standing approach for wheat improvement. <i>Dasypyrum</i><i>breviaristatum</i> species harbor novel and agronomically important genes for resistance against multi-fungal diseases. The development of new wheat-<i>D. breviaristatum</i> introgression lines offers chances for the identification of stripe rust resistance gene(s). A wheat line, D11-5, was selected from a cross between wheat line MY11 and wheat-<i>D. breviaristatum</i> partial amphiploid TDH-2. It was characterized by FISH and PCR-based molecular markers. Chromosome counting revealed that the D11-5 line shows a hexaploid set of 2n = 6x = 42 chromosomes. FISH analysis using the <i>Dasypyrum</i> repetitive sequence pDb12H as a probe demonstrated that D11-5 contained a pair of <i>D. breviaristatum</i> chromosomes, while FISH with wheat D-genomic repetitive sequences revealed that the chromosome 2D was absent in D11-5. The functional molecular markers confirmed that the introduced <i>D. breviaristatum</i> chromosomes belong to the homoeologous group 2, indicating that D11-5 was a 2V<sup>b</sup> (2D) disomic substitution line. Field resistance showed that the introduced <i>D. breviaristatum</i> chromosomes 2V<sup>b</sup> were responsible for the stripe rust resistance at the adult plant stage. FISH, C-banding, and PCR-based molecular marker analysis indicated that the chromosome 2V<sup>b</sup> of <i>D. breviaristatum</i> was completely different from the chromosome 2V of <i>D. villosum</i>. The identified wheat-<i>D. breviaristatum</i> chromosome substitution line D11-5 may be applied to produce agronomically desirable stripe rust resistance germplasm." @default.
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- W2005277635 date "2014-01-01" @default.
- W2005277635 modified "2023-09-27" @default.
- W2005277635 title "A Novel Wheat-<b><i>Dasypyrum breviaristatum </i></b>Substitution Line with Stripe Rust Resistance" @default.
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- W2005277635 doi "https://doi.org/10.1159/000366051" @default.
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