Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005283437> ?p ?o ?g. }
- W2005283437 endingPage "223" @default.
- W2005283437 startingPage "215" @default.
- W2005283437 abstract "Addition of 11-deoxycortisol to the culture medium of Curvularia lunata induced the increase of cytochrome P-450 content and steroid 11β-hydroxylase activity. The enzyme in cell-free extract produces cortisol from 11-deoxycortisol in the presence of NADPH and O2. The enzyme was partially stabilized by glycerol, 11-deoxycortisol, GSH and PMSF. The hydroxylation activity was strongly inhibited by carbon monooxid and sulfhydryl reagents. Cytochrome P-450 located on the microsomal fraction was solubilized with Triton X-100 and sodium cholate and purified to apparent homogeneity by column chromatography. The purified cytochrome P-450 (P-450lun) has a molecular mass of 60 kDa and exhibits the absorption maxium at 392 nm in the spectrum of oxidized form in the presence of 11-deoxycortisol. The reduced CO difference spectrum has a maximal peak at 448 nm. 11β-Hydroxylation of 11-deoxycortisol was reconstituted by cytochrome P-450lun, C. lunata NADPH-cytochrome P-450 reductase and DLPC in the presence of NADPH and O2 with a turnover number of 207 nmol/min per nmol of cytochrome P-450. The reductase and DLPC could be partially replaced with the enzyme purified from yeast or pig testis microsome and lipids purified from C. lunata, respectively. P-450lun catalyzes bifunctionally 11β- and 14α-hydroxylations of 11-deoxycortisol. Deoxycorticosterone, progesterone, androstenedione and testosterone are hydroxylated in the similar manner." @default.
- W2005283437 created "2016-06-24" @default.
- W2005283437 creator A5016176700 @default.
- W2005283437 creator A5020212513 @default.
- W2005283437 creator A5057493767 @default.
- W2005283437 creator A5080508987 @default.
- W2005283437 date "1993-12-01" @default.
- W2005283437 modified "2023-10-16" @default.
- W2005283437 title "Purification and properties of cytochrome P-450 (P-450lun) catalyzing steroid 11β-hydroxylation in Curvularia lunata" @default.
- W2005283437 cites W1481279942 @default.
- W2005283437 cites W1497083865 @default.
- W2005283437 cites W1509553808 @default.
- W2005283437 cites W1516776196 @default.
- W2005283437 cites W1531171254 @default.
- W2005283437 cites W153573604 @default.
- W2005283437 cites W1553433472 @default.
- W2005283437 cites W1570978364 @default.
- W2005283437 cites W15944022 @default.
- W2005283437 cites W1603978523 @default.
- W2005283437 cites W1608611152 @default.
- W2005283437 cites W1892832260 @default.
- W2005283437 cites W1939710844 @default.
- W2005283437 cites W1951469402 @default.
- W2005283437 cites W1968790539 @default.
- W2005283437 cites W1975657929 @default.
- W2005283437 cites W1976038178 @default.
- W2005283437 cites W1983393102 @default.
- W2005283437 cites W2015285864 @default.
- W2005283437 cites W2019412713 @default.
- W2005283437 cites W2031107830 @default.
- W2005283437 cites W2031995413 @default.
- W2005283437 cites W2055117832 @default.
- W2005283437 cites W2062577861 @default.
- W2005283437 cites W2083783107 @default.
- W2005283437 cites W2100837269 @default.
- W2005283437 cites W2130792052 @default.
- W2005283437 cites W2150373956 @default.
- W2005283437 cites W2151473497 @default.
- W2005283437 cites W2230713594 @default.
- W2005283437 cites W299769444 @default.
- W2005283437 cites W4366075561 @default.
- W2005283437 doi "https://doi.org/10.1016/0167-4838(93)90086-7" @default.
- W2005283437 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8268203" @default.
- W2005283437 hasPublicationYear "1993" @default.
- W2005283437 type Work @default.
- W2005283437 sameAs 2005283437 @default.
- W2005283437 citedByCount "52" @default.
- W2005283437 countsByYear W20052834372012 @default.
- W2005283437 countsByYear W20052834372013 @default.
- W2005283437 countsByYear W20052834372014 @default.
- W2005283437 countsByYear W20052834372015 @default.
- W2005283437 countsByYear W20052834372017 @default.
- W2005283437 countsByYear W20052834372018 @default.
- W2005283437 countsByYear W20052834372019 @default.
- W2005283437 countsByYear W20052834372020 @default.
- W2005283437 countsByYear W20052834372021 @default.
- W2005283437 countsByYear W20052834372022 @default.
- W2005283437 crossrefType "journal-article" @default.
- W2005283437 hasAuthorship W2005283437A5016176700 @default.
- W2005283437 hasAuthorship W2005283437A5020212513 @default.
- W2005283437 hasAuthorship W2005283437A5057493767 @default.
- W2005283437 hasAuthorship W2005283437A5080508987 @default.
- W2005283437 hasConcept C134651460 @default.
- W2005283437 hasConcept C181199279 @default.
- W2005283437 hasConcept C185592680 @default.
- W2005283437 hasConcept C2780768313 @default.
- W2005283437 hasConcept C2781109383 @default.
- W2005283437 hasConcept C43617362 @default.
- W2005283437 hasConcept C526171541 @default.
- W2005283437 hasConcept C55493867 @default.
- W2005283437 hasConcept C87644729 @default.
- W2005283437 hasConceptScore W2005283437C134651460 @default.
- W2005283437 hasConceptScore W2005283437C181199279 @default.
- W2005283437 hasConceptScore W2005283437C185592680 @default.
- W2005283437 hasConceptScore W2005283437C2780768313 @default.
- W2005283437 hasConceptScore W2005283437C2781109383 @default.
- W2005283437 hasConceptScore W2005283437C43617362 @default.
- W2005283437 hasConceptScore W2005283437C526171541 @default.
- W2005283437 hasConceptScore W2005283437C55493867 @default.
- W2005283437 hasConceptScore W2005283437C87644729 @default.
- W2005283437 hasFunder F4320320912 @default.
- W2005283437 hasIssue "2" @default.
- W2005283437 hasLocation W20052834371 @default.
- W2005283437 hasLocation W20052834372 @default.
- W2005283437 hasOpenAccess W2005283437 @default.
- W2005283437 hasPrimaryLocation W20052834371 @default.
- W2005283437 hasRelatedWork W2014397159 @default.
- W2005283437 hasRelatedWork W2017023840 @default.
- W2005283437 hasRelatedWork W2072745548 @default.
- W2005283437 hasRelatedWork W2073720507 @default.
- W2005283437 hasRelatedWork W2117945043 @default.
- W2005283437 hasRelatedWork W2336705874 @default.
- W2005283437 hasRelatedWork W2394788891 @default.
- W2005283437 hasRelatedWork W2410511421 @default.
- W2005283437 hasRelatedWork W2428725085 @default.
- W2005283437 hasRelatedWork W2462961091 @default.
- W2005283437 hasVolume "1203" @default.
- W2005283437 isParatext "false" @default.