FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005291377> ?p ?o ?g. }

• W2005291377 endingPage "85" @default.
• W2005291377 startingPage "78" @default.
• W2005291377 abstract "In fish, as well as in birds and mammals, the pineal hormone melatonin acts as a neuroendocrine transducer of photoperiod and as such has a profound influence over the timing of seasonal behavior, especially reproduction. With the aim of better understanding how melatonin may exert its influence in the fish, we have studied the distribution and characteristics of the melatonin binding sites in the brain and pituitary of the goldfish (Carassius auratus) using the ligand [2-125I]iodomelatonin and a combination of in vitro autoradiography and membrane homogenate receptor assays. Specific binding of the ligand was defined as that displaced by 1 μM melatonin. High-affinity melatonin binding sites were found to have a widespread distribution within the brain of the goldfish and measurements of the relative optical densities on X-ray films using image analysis showed that the highest concentrations were present in the visual system (optic tract, pretectal area, optic tectum), the molecular layer of the cerebellum, and the hypothalamus. No specific binding could be detected in either the pituitary gland or the olfactory bulbs. Displacement of binding by increasing concentrations of melatonin measured by comparative optical density readings on serial sections revealed an ED50 for melatonin of 10−9M. No displacement of binding by increasing concentrations of serotonin up to a concentration of 10−6 M could be detected. Binding was shown to be time dependent with maximal binding occurring after 1 h at room temperature (22°C). Addition of 1 μM melatonin after 2 h resulted in a time-dependent dissociation of ligand binding. Saturation studies carried out on crude membrane homogenates of whole brains gave rise to a straight line Scatchard plot with a KD of 69 pM and a Bmax of 14 fmol/mg protein. The widespread distribution of high-affinity melatonin binding sites in the brain of the goldfish suggests that melatonin may influence circadian and seasonal behavior in fish via numerous sites involving sensory and neuroendocrine systems but does not have a direct action on the pituitary." @default.
• W2005291377 created "2016-06-24" @default.
• W2005291377 creator A5010091165 @default.
• W2005291377 creator A5033123840 @default.
• W2005291377 creator A5054870682 @default.
• W2005291377 creator A5069823168 @default.
• W2005291377 creator A5084576649 @default.
• W2005291377 date "1991-02-01" @default.
• W2005291377 modified "2023-09-25" @default.
• W2005291377 title "Distribution of central melatonin binding sites in the goldfish (Carassius auratus)" @default.
• W2005291377 cites W1495842154 @default.
• W2005291377 cites W1966081395 @default.
• W2005291377 cites W1966498278 @default.
• W2005291377 cites W1967581397 @default.
• W2005291377 cites W1972272791 @default.
• W2005291377 cites W1976390588 @default.
• W2005291377 cites W1979122203 @default.
• W2005291377 cites W1982819563 @default.
• W2005291377 cites W1987903185 @default.
• W2005291377 cites W1993814744 @default.
• W2005291377 cites W1998398867 @default.
• W2005291377 cites W2000743194 @default.
• W2005291377 cites W2010183833 @default.
• W2005291377 cites W2012062217 @default.
• W2005291377 cites W2016604807 @default.
• W2005291377 cites W2016622707 @default.
• W2005291377 cites W2017547179 @default.
• W2005291377 cites W2024449200 @default.
• W2005291377 cites W2029135567 @default.
• W2005291377 cites W2029464665 @default.
• W2005291377 cites W2030289233 @default.
• W2005291377 cites W2039937196 @default.
• W2005291377 cites W2040554445 @default.
• W2005291377 cites W2042471476 @default.
• W2005291377 cites W2048172384 @default.
• W2005291377 cites W2054514815 @default.
• W2005291377 cites W2060652420 @default.
• W2005291377 cites W2087548420 @default.
• W2005291377 cites W2089010636 @default.
• W2005291377 cites W2089757540 @default.
• W2005291377 cites W2091196567 @default.
• W2005291377 cites W2091646716 @default.
• W2005291377 cites W2095510756 @default.
• W2005291377 cites W2123467207 @default.
• W2005291377 cites W2140850665 @default.
• W2005291377 cites W2329442555 @default.
• W2005291377 doi "https://doi.org/10.1016/1044-7431(91)90042-m" @default.
• W2005291377 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19912786" @default.
• W2005291377 hasPublicationYear "1991" @default.
• W2005291377 type Work @default.
• W2005291377 sameAs 2005291377 @default.
• W2005291377 citedByCount "60" @default.
• W2005291377 countsByYear W20052913772012 @default.
• W2005291377 countsByYear W20052913772014 @default.
• W2005291377 countsByYear W20052913772015 @default.
• W2005291377 countsByYear W20052913772021 @default.
• W2005291377 crossrefType "journal-article" @default.
• W2005291377 hasAuthorship W2005291377A5010091165 @default.
• W2005291377 hasAuthorship W2005291377A5033123840 @default.
• W2005291377 hasAuthorship W2005291377A5054870682 @default.
• W2005291377 hasAuthorship W2005291377A5069823168 @default.
• W2005291377 hasAuthorship W2005291377A5084576649 @default.
• W2005291377 hasConcept C107824862 @default.
• W2005291377 hasConcept C116569031 @default.
• W2005291377 hasConcept C118303440 @default.
• W2005291377 hasConcept C126322002 @default.
• W2005291377 hasConcept C134018914 @default.
• W2005291377 hasConcept C170493617 @default.
• W2005291377 hasConcept C2777003273 @default.
• W2005291377 hasConcept C2778182776 @default.
• W2005291377 hasConcept C2779135415 @default.
• W2005291377 hasConcept C2780059843 @default.
• W2005291377 hasConcept C2781269140 @default.
• W2005291377 hasConcept C2909208804 @default.
• W2005291377 hasConcept C2994357808 @default.
• W2005291377 hasConcept C505870484 @default.
• W2005291377 hasConcept C55493867 @default.
• W2005291377 hasConcept C71924100 @default.
• W2005291377 hasConcept C86803240 @default.
• W2005291377 hasConceptScore W2005291377C107824862 @default.
• W2005291377 hasConceptScore W2005291377C116569031 @default.
• W2005291377 hasConceptScore W2005291377C118303440 @default.
• W2005291377 hasConceptScore W2005291377C126322002 @default.
• W2005291377 hasConceptScore W2005291377C134018914 @default.
• W2005291377 hasConceptScore W2005291377C170493617 @default.
• W2005291377 hasConceptScore W2005291377C2777003273 @default.
• W2005291377 hasConceptScore W2005291377C2778182776 @default.
• W2005291377 hasConceptScore W2005291377C2779135415 @default.
• W2005291377 hasConceptScore W2005291377C2780059843 @default.
• W2005291377 hasConceptScore W2005291377C2781269140 @default.
• W2005291377 hasConceptScore W2005291377C2909208804 @default.
• W2005291377 hasConceptScore W2005291377C2994357808 @default.
• W2005291377 hasConceptScore W2005291377C505870484 @default.
• W2005291377 hasConceptScore W2005291377C55493867 @default.
• W2005291377 hasConceptScore W2005291377C71924100 @default.
• W2005291377 hasConceptScore W2005291377C86803240 @default.
• W2005291377 hasIssue "1" @default.
• W2005291377 hasLocation W20052913771 @default.

• next