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• W2005416084 abstract "Because Campylobacter jejuni is the leading cause of bacterial food-borne gastroenteritis throughout the world, there is intense effort to determine the mechanisms of infectivity associated with this bacterium. Capsular polysaccharide (CPS) has been shown to be an important virulence factor for C. jejuni and a recent study that examined the 11168V26 strain identified several phase-variable CPS modifications including an unusual O-methyl phosphoramidate (MeOPN) group on C-3 of the GalfNAc residue. In this study, we examined the MeOPN group using homo- and hetero-nuclear high-resolution magic angle spinning (HR-MAS) NMR experiments of whole bacterial cells grown on 15 NH 4 Cl-enriched media. 1 H– 31 P HSQC NMR experiments showed that the level of 15 N labeling within the MeOPN reached 80%, and a large 1 J( 15 N– 31 P) scalar coupling provided direct evidence that confirmed the structure of the MeOPN as CH 3 OP(O)(NH 2 )(OR). Because 15 N was also detected within the major outer membrane protein as well as the NAc and NGro groups of CPS, ammonium was concluded to be an important building block used in the synthesis of amino acids and glycan structures in C. jejuni. HR-MAS NMR studies of 15 N-labeled cells revealed an unanticipated level of complexity as multiple MeOPN signals were observed within 1 H- 31 P HSQC spectra for the 11168V26 and 11168H strains. While some signals originated from the MeOPN at C-3 of GalfNAc, others were attributed to a novel MeOPN located on D-glycero-α-L-gluco-heptopyranose. Together, these HR-MAS NMR findings shed light on nitrogen metabolism in C. jejuni, confirm the chemical structure of the MeOPN, and demonstrate that the modification occurs on both furanose and pyranose CPS sugars for this bacterium.Key words: Campylobacter jejuni, capsular polysaccharide, HR-MAS NMR, isotope labeling, phosphoramidate." @default.
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• W2005416084 date "2006-04-01" @default.
• W2005416084 modified "2023-10-03" @default.
• W2005416084 title "HR-MAS NMR studies of ¹⁵N-labeled cells confirm the structure of the <i>O</i>-methyl phosphoramidate CPS modification in <i>Campylobacter jejuni</i> and provide insight into its biosynthesis" @default.
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• W2005416084 doi "https://doi.org/10.1139/v06-028" @default.
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