FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005445045> ?p ?o ?g. }

• W2005445045 endingPage "894" @default.
• W2005445045 startingPage "883" @default.
• W2005445045 abstract "We found that transgenic (tg) mice stably expressing a bacterial artificial chromosome (BAC)-derived human complement receptor type 2 (CR2/CD21) gene demonstrate B cell specific hCR2 protein expression, normal B cell development and no changes in B cell subpopulations. To determine whether this BAC-encoded human CR2 (hCR2) can replace mouse CR2/CR1 in Cr2−/− mice and restore humoral immune responses to model foreign antigens (Ags), we generated hCR2+/−Cr2−/− tg mice and immunized them with sheep red blood cells (SRBC). We found that hCR2+/−Cr2−/− mice demonstrated anti-SRBC antibody (Ab) levels that were initially comparable to Cr2−/− mice after a single injection of the Ag, but then showed marked increases in anti-SRBC IgM and IgG1 levels after a second immunization. Identical results were found with a second model Ag, NP-Ficoll. To further confirm that this improvement in Ag-specific Ab production over Cr2−/− mice was indeed due to hCR2 expression, as well as to examine the effects of treating hCR2+/−Cr2−/− mice with an inhibitory anti-hCR2 monoclonal Ab (mAb) in vivo, we used mAb 171, an anti-hCR2 mAb that we have shown directly recognizes the C3d ligand binding site on hCR2. We first found that mAb 171 completely blocked hCR2-dependent co-activation of hCR2-tg B cells by anti-BCR/C3d complexes as measured in vitro by intracellular calcium influx. The i.p. injection of 1 mg of mAb 171 was then found to induce for at least three weeks only partial loss of hCR2 surface expression, without modifying B and T cell numbers or the apparent activation status of the cells. Treatment of hCR2+/−Cr2−/− mice with mAb 171 also substantially suppressed the development of anti-SRBC and anti-NP Abs following immunization with Ags. The development of this model system should allow the study of the effects of manipulating hCR2 function in vivo with potential therapeutic compounds." @default.
• W2005445045 created "2016-06-24" @default.
• W2005445045 creator A5002831713 @default.
• W2005445045 creator A5005296833 @default.
• W2005445045 creator A5040063597 @default.
• W2005445045 creator A5079260336 @default.
• W2005445045 date "2011-03-01" @default.
• W2005445045 modified "2023-10-16" @default.
• W2005445045 title "Human complement receptor type 2 (CR2/CD21) transgenic mice provide an in vivo model to study immunoregulatory effects of receptor antagonists" @default.
• W2005445045 cites W1462658072 @default.
• W2005445045 cites W148719676 @default.
• W2005445045 cites W1494539247 @default.
• W2005445045 cites W1506760954 @default.
• W2005445045 cites W1513156731 @default.
• W2005445045 cites W1517668104 @default.
• W2005445045 cites W1518090161 @default.
• W2005445045 cites W1534337168 @default.
• W2005445045 cites W1534458427 @default.
• W2005445045 cites W1545048067 @default.
• W2005445045 cites W1578564117 @default.
• W2005445045 cites W1579336178 @default.
• W2005445045 cites W1585469912 @default.
• W2005445045 cites W1587809300 @default.
• W2005445045 cites W1592353046 @default.
• W2005445045 cites W1595289904 @default.
• W2005445045 cites W1599196825 @default.
• W2005445045 cites W1604776217 @default.
• W2005445045 cites W1604837267 @default.
• W2005445045 cites W1680693957 @default.
• W2005445045 cites W1884715737 @default.
• W2005445045 cites W1900669386 @default.
• W2005445045 cites W1904609480 @default.
• W2005445045 cites W1909251229 @default.
• W2005445045 cites W1923905700 @default.
• W2005445045 cites W1928082745 @default.
• W2005445045 cites W1938707196 @default.
• W2005445045 cites W1967738350 @default.
• W2005445045 cites W1968108602 @default.
• W2005445045 cites W1970891956 @default.
• W2005445045 cites W1975913203 @default.
• W2005445045 cites W1981873656 @default.
• W2005445045 cites W1982094117 @default.
• W2005445045 cites W1984396198 @default.
• W2005445045 cites W1985837072 @default.
• W2005445045 cites W1993659659 @default.
• W2005445045 cites W2004096228 @default.
• W2005445045 cites W2007252519 @default.
• W2005445045 cites W2009313407 @default.
• W2005445045 cites W2009619688 @default.
• W2005445045 cites W2011216554 @default.
• W2005445045 cites W2013467156 @default.
• W2005445045 cites W2021248828 @default.
• W2005445045 cites W2023206654 @default.
• W2005445045 cites W2036247140 @default.
• W2005445045 cites W2038350920 @default.
• W2005445045 cites W2039096514 @default.
• W2005445045 cites W2043937927 @default.
• W2005445045 cites W2044779531 @default.
• W2005445045 cites W2046033944 @default.
• W2005445045 cites W2048567863 @default.
• W2005445045 cites W2058356662 @default.
• W2005445045 cites W2063170571 @default.
• W2005445045 cites W2064857115 @default.
• W2005445045 cites W2074409589 @default.
• W2005445045 cites W2076074155 @default.
• W2005445045 cites W2080674820 @default.
• W2005445045 cites W2081700040 @default.
• W2005445045 cites W2084183910 @default.
• W2005445045 cites W2085789847 @default.
• W2005445045 cites W2086369485 @default.
• W2005445045 cites W2086669738 @default.
• W2005445045 cites W2094886541 @default.
• W2005445045 cites W2100992053 @default.
• W2005445045 cites W2101882317 @default.
• W2005445045 cites W2104011187 @default.
• W2005445045 cites W2104237761 @default.
• W2005445045 cites W2104432354 @default.
• W2005445045 cites W2107253444 @default.
• W2005445045 cites W2110794121 @default.
• W2005445045 cites W2112249032 @default.
• W2005445045 cites W2115385241 @default.
• W2005445045 cites W2117234186 @default.
• W2005445045 cites W2123412929 @default.
• W2005445045 cites W2125672607 @default.
• W2005445045 cites W2125969570 @default.
• W2005445045 cites W2126354386 @default.
• W2005445045 cites W2127432158 @default.
• W2005445045 cites W2136600416 @default.
• W2005445045 cites W2139316669 @default.
• W2005445045 cites W2142839893 @default.
• W2005445045 cites W2145103027 @default.
• W2005445045 cites W2156878407 @default.
• W2005445045 cites W2157707857 @default.
• W2005445045 cites W2159134769 @default.
• W2005445045 cites W2168147589 @default.
• W2005445045 cites W2171440485 @default.
• W2005445045 cites W2172073591 @default.
• W2005445045 cites W2228848243 @default.

• next