Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005447223> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2005447223 endingPage "317" @default.
- W2005447223 startingPage "311" @default.
- W2005447223 abstract "Assembly-dependent trafficking is a property of many multimeric membrane protein complexes; this coupling of assembly and trafficking processes provides an important cellular quality control mechanism, ensuring that only properly folded and assembled complexes are expressed on the cell surface. In all membrane protein complexes whose trafficking is known to be assembly-dependent, at least one of the subunits contains an endoplasmic reticulum (ER) retention/retrieval signal that is shielded on subunit assembly, allowing the assembled protein complex to traffic to the plasma membrane. Under these conditions, presence of the normally retained subunit on the cell surface can be used as an indirect index of protein assembly in the ER. In this article, I describe the design of two complementary approaches (trafficking enhancement and trap assays) that can be used separately or in combination to determine whether two (or more) proteins assemble in the ER, i.e., whether they constitutively oligomerize. Both of the approaches are based on the measurement of plasma membrane-expressed proteins using antibody-mediated detection of extracellularly expressed epitopes and subsequent luminometric quantification. These methods provide a straightforward and relatively inexpensive way to assess protein-protein interactions early in the synthetic pathway." @default.
- W2005447223 created "2016-06-24" @default.
- W2005447223 creator A5039930228 @default.
- W2005447223 date "2002-08-01" @default.
- W2005447223 modified "2023-09-29" @default.
- W2005447223 title "Assembly-dependent trafficking assays in the detection of receptor–receptor interactions" @default.
- W2005447223 cites W1527134354 @default.
- W2005447223 cites W1595449036 @default.
- W2005447223 cites W1889694848 @default.
- W2005447223 cites W1915631305 @default.
- W2005447223 cites W1935980653 @default.
- W2005447223 cites W1966815718 @default.
- W2005447223 cites W1974195102 @default.
- W2005447223 cites W1975034569 @default.
- W2005447223 cites W1988395319 @default.
- W2005447223 cites W1993655108 @default.
- W2005447223 cites W2003446134 @default.
- W2005447223 cites W2004468088 @default.
- W2005447223 cites W2007051067 @default.
- W2005447223 cites W2024554932 @default.
- W2005447223 cites W2035995595 @default.
- W2005447223 cites W2040688084 @default.
- W2005447223 cites W2060202251 @default.
- W2005447223 cites W2070411747 @default.
- W2005447223 cites W2122146139 @default.
- W2005447223 cites W2130283831 @default.
- W2005447223 cites W2150716330 @default.
- W2005447223 cites W2150796852 @default.
- W2005447223 cites W2167988140 @default.
- W2005447223 cites W2172011217 @default.
- W2005447223 cites W4240544407 @default.
- W2005447223 doi "https://doi.org/10.1016/s1046-2023(02)00088-9" @default.
- W2005447223 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12217646" @default.
- W2005447223 hasPublicationYear "2002" @default.
- W2005447223 type Work @default.
- W2005447223 sameAs 2005447223 @default.
- W2005447223 citedByCount "31" @default.
- W2005447223 countsByYear W20054472232014 @default.
- W2005447223 countsByYear W20054472232015 @default.
- W2005447223 countsByYear W20054472232017 @default.
- W2005447223 countsByYear W20054472232021 @default.
- W2005447223 crossrefType "journal-article" @default.
- W2005447223 hasAuthorship W2005447223A5039930228 @default.
- W2005447223 hasConcept C104292427 @default.
- W2005447223 hasConcept C104317684 @default.
- W2005447223 hasConcept C12554922 @default.
- W2005447223 hasConcept C144647389 @default.
- W2005447223 hasConcept C158617107 @default.
- W2005447223 hasConcept C170493617 @default.
- W2005447223 hasConcept C185592680 @default.
- W2005447223 hasConcept C2778106830 @default.
- W2005447223 hasConcept C41625074 @default.
- W2005447223 hasConcept C55493867 @default.
- W2005447223 hasConcept C57581600 @default.
- W2005447223 hasConcept C86803240 @default.
- W2005447223 hasConcept C95444343 @default.
- W2005447223 hasConceptScore W2005447223C104292427 @default.
- W2005447223 hasConceptScore W2005447223C104317684 @default.
- W2005447223 hasConceptScore W2005447223C12554922 @default.
- W2005447223 hasConceptScore W2005447223C144647389 @default.
- W2005447223 hasConceptScore W2005447223C158617107 @default.
- W2005447223 hasConceptScore W2005447223C170493617 @default.
- W2005447223 hasConceptScore W2005447223C185592680 @default.
- W2005447223 hasConceptScore W2005447223C2778106830 @default.
- W2005447223 hasConceptScore W2005447223C41625074 @default.
- W2005447223 hasConceptScore W2005447223C55493867 @default.
- W2005447223 hasConceptScore W2005447223C57581600 @default.
- W2005447223 hasConceptScore W2005447223C86803240 @default.
- W2005447223 hasConceptScore W2005447223C95444343 @default.
- W2005447223 hasIssue "4" @default.
- W2005447223 hasLocation W20054472231 @default.
- W2005447223 hasLocation W20054472232 @default.
- W2005447223 hasOpenAccess W2005447223 @default.
- W2005447223 hasPrimaryLocation W20054472231 @default.
- W2005447223 hasRelatedWork W1181096670 @default.
- W2005447223 hasRelatedWork W1977146061 @default.
- W2005447223 hasRelatedWork W1980496425 @default.
- W2005447223 hasRelatedWork W2110810002 @default.
- W2005447223 hasRelatedWork W2114616822 @default.
- W2005447223 hasRelatedWork W2140225001 @default.
- W2005447223 hasRelatedWork W2150351373 @default.
- W2005447223 hasRelatedWork W2176649486 @default.
- W2005447223 hasRelatedWork W2407818549 @default.
- W2005447223 hasRelatedWork W3027428660 @default.
- W2005447223 hasVolume "27" @default.
- W2005447223 isParatext "false" @default.
- W2005447223 isRetracted "false" @default.
- W2005447223 magId "2005447223" @default.
- W2005447223 workType "article" @default.