FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005452431> ?p ?o ?g. }

• W2005452431 endingPage "561" @default.
• W2005452431 startingPage "551" @default.
• W2005452431 abstract "IF3 has a fidelity function in the initiation of translation, inducing the dissociation of fMet-tRNAfMet from the 30 S initiation complexes (30SIC) containing a non-canonical initiation triplet (e.g. AUU) in place of a canonical initiation triplet (e.g., AUG). IF2 has a complementary role, selectively promoting initiator tRNA binding to the ribosome. Here, we used parallel rapid kinetics measurements of GTP hydrolysis, Pi release, light-scattering, and changes in intensities of fluorophore-labeled IF2 and fMet-tRNAfMet to determine the effects on both 30SIC formation and 30SIC conversion to 70 S initiation complexes (70SIC) of (a) substituting AUG with AUU, and/or (b) omitting IF3, and/or (c) replacing GTP with the non-hydrolyzable analog GDPCP. We demonstrate that the presence or absence of IF3 has, at most, minor effects on the rate of 30SIC formation using either AUG or AUU as the initiation codon, and conclude that the high affinity of IF2 for both 30 S subunit and initiator tRNA overrides any perturbation of the codon–anticodon interaction resulting from AUU for AUG substitution. In contrast, replacement of AUG by AUU leads to a dramatic reduction in the rate of 70SIC formation from 30SIC upon addition of 50 S subunits. Interpreting our results in the framework of a quantitative kinetic scheme leads to the conclusion that, within the overall process of 70SIC formation, the step most affected by substituting AUU for AUG involves the conversion of an initially labile 70 S ribosome into a more stable complex. In the absence of IF3, the difference between AUG and AUU largely disappears, with each initiation codon affording rapid 70SIC formation, leading to the hypothesis that it is the rate of IF3 dissociation from the 70 S ribosome during IC70S formation that is critical to its fidelity function." @default.
• W2005452431 created "2016-06-24" @default.
• W2005452431 creator A5000742587 @default.
• W2005452431 creator A5001069964 @default.
• W2005452431 creator A5001791914 @default.
• W2005452431 creator A5036657037 @default.
• W2005452431 creator A5083810937 @default.
• W2005452431 date "2007-10-01" @default.
• W2005452431 modified "2023-10-16" @default.
• W2005452431 title "The Translational Fidelity Function of IF3 During Transition from the 30 S Initiation Complex to the 70 S Initiation Complex" @default.
• W2005452431 cites W1552186322 @default.
• W2005452431 cites W1554525826 @default.
• W2005452431 cites W1899875913 @default.
• W2005452431 cites W1932391635 @default.
• W2005452431 cites W1965178458 @default.
• W2005452431 cites W1965383448 @default.
• W2005452431 cites W1972912399 @default.
• W2005452431 cites W1977243327 @default.
• W2005452431 cites W1986763488 @default.
• W2005452431 cites W1992821647 @default.
• W2005452431 cites W1993128416 @default.
• W2005452431 cites W2007890475 @default.
• W2005452431 cites W2016412131 @default.
• W2005452431 cites W2018658699 @default.
• W2005452431 cites W2025856525 @default.
• W2005452431 cites W2033414595 @default.
• W2005452431 cites W2037213262 @default.
• W2005452431 cites W2037679031 @default.
• W2005452431 cites W2038864352 @default.
• W2005452431 cites W2041188328 @default.
• W2005452431 cites W2043991638 @default.
• W2005452431 cites W2045491774 @default.
• W2005452431 cites W2049997941 @default.
• W2005452431 cites W2059501803 @default.
• W2005452431 cites W2066896073 @default.
• W2005452431 cites W2068628797 @default.
• W2005452431 cites W2071390057 @default.
• W2005452431 cites W2071877490 @default.
• W2005452431 cites W2073354904 @default.
• W2005452431 cites W2081540493 @default.
• W2005452431 cites W2085211780 @default.
• W2005452431 cites W2091336733 @default.
• W2005452431 cites W2095598198 @default.
• W2005452431 cites W2103192963 @default.
• W2005452431 cites W2112130400 @default.
• W2005452431 cites W2118391959 @default.
• W2005452431 cites W2129561341 @default.
• W2005452431 cites W2134660959 @default.
• W2005452431 cites W2149357806 @default.
• W2005452431 cites W2171108120 @default.
• W2005452431 doi "https://doi.org/10.1016/j.jmb.2007.07.031" @default.
• W2005452431 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2083563" @default.
• W2005452431 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17868695" @default.
• W2005452431 hasPublicationYear "2007" @default.
• W2005452431 type Work @default.
• W2005452431 sameAs 2005452431 @default.
• W2005452431 citedByCount "66" @default.
• W2005452431 countsByYear W20054524312012 @default.
• W2005452431 countsByYear W20054524312013 @default.
• W2005452431 countsByYear W20054524312014 @default.
• W2005452431 countsByYear W20054524312015 @default.
• W2005452431 countsByYear W20054524312016 @default.
• W2005452431 countsByYear W20054524312017 @default.
• W2005452431 countsByYear W20054524312018 @default.
• W2005452431 countsByYear W20054524312019 @default.
• W2005452431 countsByYear W20054524312020 @default.
• W2005452431 countsByYear W20054524312021 @default.
• W2005452431 countsByYear W20054524312022 @default.
• W2005452431 countsByYear W20054524312023 @default.
• W2005452431 crossrefType "journal-article" @default.
• W2005452431 hasAuthorship W2005452431A5000742587 @default.
• W2005452431 hasAuthorship W2005452431A5001069964 @default.
• W2005452431 hasAuthorship W2005452431A5001791914 @default.
• W2005452431 hasAuthorship W2005452431A5036657037 @default.
• W2005452431 hasAuthorship W2005452431A5083810937 @default.
• W2005452431 hasBestOaLocation W20054524312 @default.
• W2005452431 hasConcept C104317684 @default.
• W2005452431 hasConcept C105580179 @default.
• W2005452431 hasConcept C118716 @default.
• W2005452431 hasConcept C121332964 @default.
• W2005452431 hasConcept C12554922 @default.
• W2005452431 hasConcept C148898269 @default.
• W2005452431 hasConcept C149364088 @default.
• W2005452431 hasConcept C153957851 @default.
• W2005452431 hasConcept C176990463 @default.
• W2005452431 hasConcept C181199279 @default.
• W2005452431 hasConcept C185592680 @default.
• W2005452431 hasConcept C4718897 @default.
• W2005452431 hasConcept C512185932 @default.
• W2005452431 hasConcept C55493867 @default.
• W2005452431 hasConcept C62493599 @default.
• W2005452431 hasConcept C62520636 @default.
• W2005452431 hasConcept C67705224 @default.
• W2005452431 hasConcept C71240020 @default.
• W2005452431 hasConcept C8010536 @default.
• W2005452431 hasConcept C86803240 @default.
• W2005452431 hasConcept C88478588 @default.
• W2005452431 hasConcept C93734116 @default.

• next