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- W2005455989 abstract "MicroRNAs (miR) are small pieces of RNA that have the ability to post-transcriptionally regulate protein expression. Many miR have been shown to be differentially expressed in the cortex of AD patients and seemingly play a role in AD pathogenesis, rendering them a highly interesting target. In this study we aimed to evaluate whether miR can be measured in cerebrospinal fluid (CSF) and to ascertain whether miR are differentially expressed in CSF of Alzheimer's disease (AD) patients using a data-driven qRT-PCR approach. RNA was isolated from 2 ml CSF of 19 AD patients and 19 age and sex-matched controls using the mirVana Paris isolation kit (Ambion). miR were quantified by means of qRT-PCR using Taqman Array MicroRNA Cards A and B (v3.0; Applied Biosystems) according to the megaplex protocol. Mean Ct value of all miR present in one plate was used as endogenous control. Relative quantification (RQ) was calculated as 2-ddCt. On average 107 ± 44 out of 776 miR (14%)were detected in CSF using megaplex array analysis. Abundance was very low with an average Ct value of 33 ± 1 in all detected miR. Exploratory analysis showed a correlation between age and number of miR detected (r = -0.41, P<0.05) and between age and mean Ct value of all detected miR (r = 0.47, P <0.01). Because of these strong correlations, we performed separate between group comparisons for older and younger patients (cut-off 65 years). In the early-onset AD group 13 miR were differentially expressed (P <0.05). In the late-onset AD group 6 other miR were differentially expressed (P <0.05). We have shown that it is possible to measure miR in CSF, suggesting a potential future use as biomarkers in living patients. Several miR seem to be differentially expressed in CSF of AD patients. Further investigation is warranted, especially since accumulating evidence suggests miR expression plays an important role in AD pathogenesis." @default.
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- W2005455989 date "2012-07-01" @default.
- W2005455989 modified "2023-09-27" @default.
- W2005455989 title "P2-034: miRNA expression in cerebrospinal fluid of Alzheimer's disease patients" @default.
- W2005455989 doi "https://doi.org/10.1016/j.jalz.2012.05.737" @default.
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