FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005459548> ?p ?o ?g. }

• W2005459548 endingPage "441" @default.
• W2005459548 startingPage "430" @default.
• W2005459548 abstract "Previous work from our laboratory has established bovine hippocampal membranes as a convenient natural source for studying neuronal receptors such as the G-protein coupled serotonin1A receptor. In this paper, we have explored the organization and dynamics of bovine hippocampal membranes using environment-sensitive and differentially localized fluorescent probes NBD-PE and NBD-cholesterol, utilizing wavelength-selective and time-resolved fluorescence measurements. The NBD group in NBD-PE is localized at the membrane interface while in NBD-cholesterol it is localized deeper in the membrane. Our results show that native hippocampal membranes offer considerable motional restriction as evidenced from red edge excitation shift of NBD probes. However, this effect progressively decreases with increasing cholesterol depletion in the case of NBD-cholesterol, possibly indicating a reduction in membrane heterogeneity. In contrast, REES of NBD-PE in hippocampal membranes does not show any significant change upon cholesterol depletion indicating relative lack of sensitivity of the membrane interface to cholesterol depletion. These observations are supported by changes in fluorescence polarization with cholesterol depletion. Taken together, these results imply that the deeper hydrocarbon region of the hippocampal membrane is more sensitive to changes in membrane organization and dynamics due to cholesterol depletion than the interfacial region. The motional restriction in native membranes is maintained even in the absence of proteins. The fluorescence lifetimes of both the NBD probes show slight reduction upon cholesterol depletion indicating a change in micro-environmental polarity possibly due to water penetration. These results are relevant in understanding the complex organization of hippocampal membranes and could have possible functional implications." @default.
• W2005459548 created "2016-06-24" @default.
• W2005459548 creator A5047952576 @default.
• W2005459548 creator A5063835467 @default.
• W2005459548 creator A5079748663 @default.
• W2005459548 creator A5080833052 @default.
• W2005459548 date "2006-01-01" @default.
• W2005459548 modified "2023-10-18" @default.
• W2005459548 title "Monitoring the organization and dynamics of bovine hippocampal membranes utilizing differentially localized fluorescent membrane probes" @default.
• W2005459548 cites W145618170 @default.
• W2005459548 cites W157189559 @default.
• W2005459548 cites W1972519777 @default.
• W2005459548 cites W1975783279 @default.
• W2005459548 cites W1983351621 @default.
• W2005459548 cites W1990662818 @default.
• W2005459548 cites W1994252543 @default.
• W2005459548 cites W2002990498 @default.
• W2005459548 cites W2003742510 @default.
• W2005459548 cites W2010122297 @default.
• W2005459548 cites W2023629524 @default.
• W2005459548 cites W2025826079 @default.
• W2005459548 cites W2031113704 @default.
• W2005459548 cites W2031687154 @default.
• W2005459548 cites W2032496660 @default.
• W2005459548 cites W2039311957 @default.
• W2005459548 cites W2049848440 @default.
• W2005459548 cites W2064539835 @default.
• W2005459548 cites W2066353340 @default.
• W2005459548 cites W2068251898 @default.
• W2005459548 cites W2072578069 @default.
• W2005459548 cites W2078397595 @default.
• W2005459548 cites W2082271652 @default.
• W2005459548 cites W2085525100 @default.
• W2005459548 cites W2089880141 @default.
• W2005459548 cites W2094753712 @default.
• W2005459548 cites W2099559490 @default.
• W2005459548 cites W2102681754 @default.
• W2005459548 cites W2103709374 @default.
• W2005459548 cites W2111930403 @default.
• W2005459548 cites W2129995310 @default.
• W2005459548 cites W2152311269 @default.
• W2005459548 cites W4232534952 @default.
• W2005459548 cites W4255678540 @default.
• W2005459548 doi "https://doi.org/10.1080/09687860600803223" @default.
• W2005459548 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17060160" @default.
• W2005459548 hasPublicationYear "2006" @default.
• W2005459548 type Work @default.
• W2005459548 sameAs 2005459548 @default.
• W2005459548 citedByCount "10" @default.
• W2005459548 countsByYear W20054595482012 @default.
• W2005459548 countsByYear W20054595482014 @default.
• W2005459548 countsByYear W20054595482015 @default.
• W2005459548 countsByYear W20054595482019 @default.
• W2005459548 crossrefType "journal-article" @default.
• W2005459548 hasAuthorship W2005459548A5047952576 @default.
• W2005459548 hasAuthorship W2005459548A5063835467 @default.
• W2005459548 hasAuthorship W2005459548A5079748663 @default.
• W2005459548 hasAuthorship W2005459548A5080833052 @default.
• W2005459548 hasConcept C111357860 @default.
• W2005459548 hasConcept C121332964 @default.
• W2005459548 hasConcept C12554922 @default.
• W2005459548 hasConcept C148762608 @default.
• W2005459548 hasConcept C169760540 @default.
• W2005459548 hasConcept C185592680 @default.
• W2005459548 hasConcept C2778163477 @default.
• W2005459548 hasConcept C2780727160 @default.
• W2005459548 hasConcept C41625074 @default.
• W2005459548 hasConcept C55493867 @default.
• W2005459548 hasConcept C62520636 @default.
• W2005459548 hasConcept C86803240 @default.
• W2005459548 hasConcept C91881484 @default.
• W2005459548 hasConceptScore W2005459548C111357860 @default.
• W2005459548 hasConceptScore W2005459548C121332964 @default.
• W2005459548 hasConceptScore W2005459548C12554922 @default.
• W2005459548 hasConceptScore W2005459548C148762608 @default.
• W2005459548 hasConceptScore W2005459548C169760540 @default.
• W2005459548 hasConceptScore W2005459548C185592680 @default.
• W2005459548 hasConceptScore W2005459548C2778163477 @default.
• W2005459548 hasConceptScore W2005459548C2780727160 @default.
• W2005459548 hasConceptScore W2005459548C41625074 @default.
• W2005459548 hasConceptScore W2005459548C55493867 @default.
• W2005459548 hasConceptScore W2005459548C62520636 @default.
• W2005459548 hasConceptScore W2005459548C86803240 @default.
• W2005459548 hasConceptScore W2005459548C91881484 @default.
• W2005459548 hasIssue "5" @default.
• W2005459548 hasLocation W20054595481 @default.
• W2005459548 hasLocation W20054595482 @default.
• W2005459548 hasOpenAccess W2005459548 @default.
• W2005459548 hasPrimaryLocation W20054595481 @default.
• W2005459548 hasRelatedWork W2000148018 @default.
• W2005459548 hasRelatedWork W2037559848 @default.
• W2005459548 hasRelatedWork W2038680277 @default.
• W2005459548 hasRelatedWork W2048400417 @default.
• W2005459548 hasRelatedWork W2064011811 @default.
• W2005459548 hasRelatedWork W2140725547 @default.
• W2005459548 hasRelatedWork W2155593527 @default.
• W2005459548 hasRelatedWork W2169178224 @default.
• W2005459548 hasRelatedWork W2977330715 @default.

• next