FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005470982> ?p ?o ?g. }

• W2005470982 endingPage "17" @default.
• W2005470982 startingPage "1" @default.
• W2005470982 abstract "In this study we examined the passive biocompatibility of a three-dimensional microelectrode array (MEA), designed to be coupled to organotypic brain slice cultures for multisite recording of electrophysiological signals. Hippocampal (and corticostriatal) brain slices from 1-week-old (and newborn) rats were grown for 4-8 weeks on the perforated silicon chips with silicon nitride surfaces and 40 microm sized holes and compared with corresponding tissue slices grown on conventional semiporous membranes. In terms of preservation of the basic cellular and connective organization, as visualized by Nissl staining, Timm sulphide silver-staining, microtubule-associated protein 2 (MAP2) and glial fibrillary acidic protein (GFAP) immunostaining, the slice cultures grown on chips did not differ from conventionally grown slice cultures. Neither were there any signs of astrogliosis or neurodegeneration around the upper recording part of the 47-microm-high platinum-tip electrodes. Slice cultures grown on a separate set of chips with platinum instead of silicon nitride surfaces also displayed normal MAP2 and GFAP immunostaining. The width of the GFAP-rich zone (glia limitans) at the bottom surface of the slice cultures was the same ( approximately 20 microm) in cultures grown on chips with silicon nitride and platinum surfaces and on conventional insert membranes. The slice cultures grown on chips maintained a normal, subfield differentiated susceptibility to the glutamate receptor agonist N-methyl-D-aspartate (NMDA) and the neurotoxin trimethyltin (TMT), as demonstrated by the cellular uptake of propidium iodide (PI), which was used as a reproducible and quantifiable marker for neuronal degeneration. We conclude that organotypic brain slice cultures can grow on silicon-based three-dimensional microelectrode arrays and develop normally with display of normal subfield differentiated susceptibilities to known excito- and neurotoxins. From this it is anticipated that the set-up, designed for recording of electrophysiological parameters, can be used for long-term studies of defined neuronal networks and provide valuable information on both normal, neurotoxicological and neuropathological conditions." @default.
• W2005470982 created "2016-06-24" @default.
• W2005470982 creator A5018346224 @default.
• W2005470982 creator A5019507056 @default.
• W2005470982 creator A5023573690 @default.
• W2005470982 creator A5033122852 @default.
• W2005470982 creator A5062444354 @default.
• W2005470982 date "2001-03-01" @default.
• W2005470982 modified "2023-10-16" @default.
• W2005470982 title "Biocompatibility of silicon-based arrays of electrodes coupled to organotypic hippocampal brain slice cultures" @default.
• W2005470982 cites W1567544633 @default.
• W2005470982 cites W1599155465 @default.
• W2005470982 cites W1964294097 @default.
• W2005470982 cites W1970377013 @default.
• W2005470982 cites W1972671198 @default.
• W2005470982 cites W1975188658 @default.
• W2005470982 cites W1975529898 @default.
• W2005470982 cites W1989346176 @default.
• W2005470982 cites W1992516396 @default.
• W2005470982 cites W1994612125 @default.
• W2005470982 cites W1999201911 @default.
• W2005470982 cites W2002816215 @default.
• W2005470982 cites W2005525115 @default.
• W2005470982 cites W2006376420 @default.
• W2005470982 cites W2010085531 @default.
• W2005470982 cites W2010472439 @default.
• W2005470982 cites W2011713014 @default.
• W2005470982 cites W2022316962 @default.
• W2005470982 cites W2025506306 @default.
• W2005470982 cites W2031724434 @default.
• W2005470982 cites W2038143664 @default.
• W2005470982 cites W2038733241 @default.
• W2005470982 cites W2043985705 @default.
• W2005470982 cites W2048724759 @default.
• W2005470982 cites W2054844454 @default.
• W2005470982 cites W2073667820 @default.
• W2005470982 cites W2079054696 @default.
• W2005470982 cites W2088609449 @default.
• W2005470982 cites W2092093968 @default.
• W2005470982 cites W2092895968 @default.
• W2005470982 cites W2161754062 @default.
• W2005470982 cites W2170342175 @default.
• W2005470982 cites W2171617589 @default.
• W2005470982 doi "https://doi.org/10.1016/s0006-8993(00)03304-7" @default.
• W2005470982 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11277967" @default.
• W2005470982 hasPublicationYear "2001" @default.
• W2005470982 type Work @default.
• W2005470982 sameAs 2005470982 @default.
• W2005470982 citedByCount "68" @default.
• W2005470982 countsByYear W20054709822012 @default.
• W2005470982 countsByYear W20054709822013 @default.
• W2005470982 countsByYear W20054709822014 @default.
• W2005470982 countsByYear W20054709822015 @default.
• W2005470982 countsByYear W20054709822016 @default.
• W2005470982 countsByYear W20054709822017 @default.
• W2005470982 countsByYear W20054709822018 @default.
• W2005470982 countsByYear W20054709822019 @default.
• W2005470982 countsByYear W20054709822020 @default.
• W2005470982 countsByYear W20054709822021 @default.
• W2005470982 countsByYear W20054709822022 @default.
• W2005470982 countsByYear W20054709822023 @default.
• W2005470982 crossrefType "journal-article" @default.
• W2005470982 hasAuthorship W2005470982A5018346224 @default.
• W2005470982 hasAuthorship W2005470982A5019507056 @default.
• W2005470982 hasAuthorship W2005470982A5023573690 @default.
• W2005470982 hasAuthorship W2005470982A5033122852 @default.
• W2005470982 hasAuthorship W2005470982A5062444354 @default.
• W2005470982 hasBestOaLocation W20054709822 @default.
• W2005470982 hasConcept C12554922 @default.
• W2005470982 hasConcept C16945437 @default.
• W2005470982 hasConcept C169760540 @default.
• W2005470982 hasConcept C185592680 @default.
• W2005470982 hasConcept C190283241 @default.
• W2005470982 hasConcept C192562407 @default.
• W2005470982 hasConcept C203014093 @default.
• W2005470982 hasConcept C204232928 @default.
• W2005470982 hasConcept C2775934118 @default.
• W2005470982 hasConcept C2777542381 @default.
• W2005470982 hasConcept C2780495277 @default.
• W2005470982 hasConcept C29694410 @default.
• W2005470982 hasConcept C31573885 @default.
• W2005470982 hasConcept C4224716 @default.
• W2005470982 hasConcept C529278444 @default.
• W2005470982 hasConcept C55493867 @default.
• W2005470982 hasConcept C86803240 @default.
• W2005470982 hasConceptScore W2005470982C12554922 @default.
• W2005470982 hasConceptScore W2005470982C16945437 @default.
• W2005470982 hasConceptScore W2005470982C169760540 @default.
• W2005470982 hasConceptScore W2005470982C185592680 @default.
• W2005470982 hasConceptScore W2005470982C190283241 @default.
• W2005470982 hasConceptScore W2005470982C192562407 @default.
• W2005470982 hasConceptScore W2005470982C203014093 @default.
• W2005470982 hasConceptScore W2005470982C204232928 @default.
• W2005470982 hasConceptScore W2005470982C2775934118 @default.
• W2005470982 hasConceptScore W2005470982C2777542381 @default.
• W2005470982 hasConceptScore W2005470982C2780495277 @default.
• W2005470982 hasConceptScore W2005470982C29694410 @default.
• W2005470982 hasConceptScore W2005470982C31573885 @default.

• next