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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005472509> ?p ?o ?g. }

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• W2005472509 abstract "Protein detection is a common yet time-intensive task in many laboratories. Here we report a protocol that makes use of cold microwave technology to reduce the total processing time to less than 1 h with dot and Western blot applications while yielding lower background noise at similar signal strength when compared with conventional protocols. With dot blots, the time savings was accompanied by a decrease in reagent use. With Western blots, the visibility of prestained markers was maintained, in stark contrast to conventional procedures. Experiments kept at a constant temperature of 21 degrees C support the existence of a microwave radiation effect, whereas an additional thermal effect is noted when the temperature is increased to 37 degrees C from ambient. Microwave-assisted dot blotting is suggested as an effective way of facilitating large-scale screening of expressed proteins." @default.
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• W2005472509 date "2008-04-01" @default.
• W2005472509 modified "2023-10-04" @default.
• W2005472509 title "Protein screening using cold microwave technology" @default.
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• W2005472509 doi "https://doi.org/10.1016/j.ab.2008.01.019" @default.
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