Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005476078> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2005476078 endingPage "791" @default.
- W2005476078 startingPage "787" @default.
- W2005476078 abstract "L-Proline is oxidized to pyrroline-5-carboxylic acid in intact plant mitochondria by a proline dehydrogenase (EC 1.4.3) that is bound to the matrix side of the inner mitochondrial membrane (TE Elthon, CR Stewart [1981] Plant Physiol 67: 780-784). This investigation reports the first solubilization of the L-proline dehydrogenase (PDH) from plant mitochondria. The supernatant from NP-40-treated etiolated shoot mitochondria of maize, Zea mays L., reduced iodonitrotetrazolium violet in a proline dependent manner. The pH optimum for this activity was 8. The apparent K m for proline was 6.6 millimolar. When supplied with proline, this solubilized PDH activity also synthesized pyrroline-5-carboxylic acid. The PDH activity was inhibited in vitro by 300 millimolar potassium chloride but not by 300 millimolar potassium acetate. The PDH activity had a molecular mass that was greater than 150 kilodaltons. Mitochondria were prepared from etiolated shoots grown in 100% water-saturated vermiculite (control) and 16% water-saturated vermiculite (stress). The specific activity of solubilized PDH from the stress treatment was 11% of the same activity from the control treatment. Oxygen uptake in the presence of proline and ADP (state 3 proline oxidation) by mitochondria from the stress treatment was 25% of the same rate by mitochondria from the control treatment. Mitochondria were also prepared 16 hours after rewatering the seedlings growing in the stress treatment. Both the solubilized PDH specific activity and state 3 proline oxidation returned to the control levels. The specific activities of the NAD+-dependent pyrroline-5-carboxylic acid dehydrogenase and cytochrome c oxidase in the solubilized preparations were unaffected by these stress and recovery treatments. Oxygen uptake rates by intact mitochondria in the presence of ADP and NADH, succinate or malate-pyruvate were also unaffected by these treatments." @default.
- W2005476078 created "2016-06-24" @default.
- W2005476078 creator A5009794402 @default.
- W2005476078 creator A5047062612 @default.
- W2005476078 date "1991-03-01" @default.
- W2005476078 modified "2023-09-27" @default.
- W2005476078 title "Solubilization of a Proline Dehydrogenase from Maize (<i>Zea mays</i> L.) Mitochondria" @default.
- W2005476078 cites W1703421765 @default.
- W2005476078 cites W1961922871 @default.
- W2005476078 cites W1972777699 @default.
- W2005476078 cites W1976400172 @default.
- W2005476078 cites W1980541510 @default.
- W2005476078 cites W1987588304 @default.
- W2005476078 cites W2001498200 @default.
- W2005476078 cites W2006680867 @default.
- W2005476078 cites W2066414091 @default.
- W2005476078 cites W2078704305 @default.
- W2005476078 cites W75870934 @default.
- W2005476078 doi "https://doi.org/10.1104/pp.95.3.787" @default.
- W2005476078 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1077606" @default.
- W2005476078 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16668054" @default.
- W2005476078 hasPublicationYear "1991" @default.
- W2005476078 type Work @default.
- W2005476078 sameAs 2005476078 @default.
- W2005476078 citedByCount "87" @default.
- W2005476078 countsByYear W20054760782012 @default.
- W2005476078 countsByYear W20054760782013 @default.
- W2005476078 countsByYear W20054760782014 @default.
- W2005476078 countsByYear W20054760782015 @default.
- W2005476078 countsByYear W20054760782016 @default.
- W2005476078 countsByYear W20054760782017 @default.
- W2005476078 countsByYear W20054760782018 @default.
- W2005476078 countsByYear W20054760782019 @default.
- W2005476078 countsByYear W20054760782020 @default.
- W2005476078 countsByYear W20054760782021 @default.
- W2005476078 crossrefType "journal-article" @default.
- W2005476078 hasAuthorship W2005476078A5009794402 @default.
- W2005476078 hasAuthorship W2005476078A5047062612 @default.
- W2005476078 hasBestOaLocation W20054760781 @default.
- W2005476078 hasConcept C104317684 @default.
- W2005476078 hasConcept C106827334 @default.
- W2005476078 hasConcept C178790620 @default.
- W2005476078 hasConcept C185592680 @default.
- W2005476078 hasConcept C2779815552 @default.
- W2005476078 hasConcept C2781132150 @default.
- W2005476078 hasConcept C28859421 @default.
- W2005476078 hasConcept C515207424 @default.
- W2005476078 hasConcept C55493867 @default.
- W2005476078 hasConcept C69305403 @default.
- W2005476078 hasConcept C86803240 @default.
- W2005476078 hasConceptScore W2005476078C104317684 @default.
- W2005476078 hasConceptScore W2005476078C106827334 @default.
- W2005476078 hasConceptScore W2005476078C178790620 @default.
- W2005476078 hasConceptScore W2005476078C185592680 @default.
- W2005476078 hasConceptScore W2005476078C2779815552 @default.
- W2005476078 hasConceptScore W2005476078C2781132150 @default.
- W2005476078 hasConceptScore W2005476078C28859421 @default.
- W2005476078 hasConceptScore W2005476078C515207424 @default.
- W2005476078 hasConceptScore W2005476078C55493867 @default.
- W2005476078 hasConceptScore W2005476078C69305403 @default.
- W2005476078 hasConceptScore W2005476078C86803240 @default.
- W2005476078 hasIssue "3" @default.
- W2005476078 hasLocation W20054760781 @default.
- W2005476078 hasLocation W20054760782 @default.
- W2005476078 hasLocation W20054760783 @default.
- W2005476078 hasOpenAccess W2005476078 @default.
- W2005476078 hasPrimaryLocation W20054760781 @default.
- W2005476078 hasRelatedWork W1532372971 @default.
- W2005476078 hasRelatedWork W1939892327 @default.
- W2005476078 hasRelatedWork W2000885990 @default.
- W2005476078 hasRelatedWork W2008162817 @default.
- W2005476078 hasRelatedWork W2079366773 @default.
- W2005476078 hasRelatedWork W2115551026 @default.
- W2005476078 hasRelatedWork W2150917915 @default.
- W2005476078 hasRelatedWork W3117961322 @default.
- W2005476078 hasRelatedWork W3139900647 @default.
- W2005476078 hasRelatedWork W4313299154 @default.
- W2005476078 hasVolume "95" @default.
- W2005476078 isParatext "false" @default.
- W2005476078 isRetracted "false" @default.
- W2005476078 magId "2005476078" @default.
- W2005476078 workType "article" @default.