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- W2005508512 abstract "The radio enzyme assay for histamine based on the transmethylation with purified histamine-N-methyltransferase and utilizing [3H-methyl]-S-adenosylmethionine as the methyl donor has been optimized to measure low histamine concentrations, for example in plasma. The pH-optimum for the assay is pH 8.3 in Tris-glycine buffer at 20°C. An incubation time of 90 min is necessary using an enzyme concentration of 5.8 μg/ml. EDTA and dithiothreitol were included in the assay to keep the histamine-N-methyltransferase active as agents that oxidize -SH groups were found to be inhibitory to the reaction. The present assay is sensitive to about 0.5 nmol/l of histamine in a sample volume of 50 μl (about 3 pg/sample)." @default.
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- W2005508512 date "1984-11-01" @default.
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- W2005508512 title "Histamine N-methyltransferase preparation used for histamine assay contains endogenous histamine" @default.
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- W2005508512 doi "https://doi.org/10.1016/0009-8981(84)90087-1" @default.
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