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• W2005539780 endingPage "2574" @default.
• W2005539780 startingPage "2561" @default.
• W2005539780 abstract "Previous research in our laboratory comparing the three-dimensional structural elements of two highly homologous alcohol dehydrogenases, one from the mesophile Clostridium beijerinckii (CbADH) and the other from the extreme thermophile Thermoanaerobacter brockii (TbADH), suggested that in the thermophilic enzyme, an extra intrasubunit ion pair (Glu224-Lys254) and a short ion-pair network (Lys257-Asp237-Arg304-Glu165) at the intersubunit interface might contribute to the extreme thermal stability of TbADH. In the present study, we used site-directed mutagenesis to replace these structurally strategic residues in CbADH with the corresponding amino acids from TbADH, and we determined the effect of such replacements on the thermal stability of CbADH. Mutations in the intrasubunit ion pair region increased thermostability in the single mutant S254K- and in the double mutant V224E/S254K-CbADH, but not in the single mutant V224E-CbADH. Both single amino acid replacements, M304R- and Q165E-CbADH, in the region of the intersubunit ion pair network augmented thermal stability, with an additive effect in the double mutant M304R/Q165E-CbADH. To investigate the precise mechanism by which such mutations alter the molecular structure of CbADH to achieve enhanced thermostability, we constructed a quadruple mutant V224E/S254K/Q165E/M304R-CbADH and solved its three-dimensional structure. The overall results indicate that the amino acid substitutions in CbADH mutants with enhanced thermal stability reinforce the quaternary structure of the enzyme by formation of an extended network of intersubunit ion pairs and salt bridges, mediated by water molecules, and by forming a new intrasubunit salt bridge." @default.
• W2005539780 created "2016-06-24" @default.
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• W2005539780 date "2002-11-01" @default.
• W2005539780 modified "2023-10-11" @default.
• W2005539780 title "Structural basis for the enhanced thermal stability of alcohol dehydrogenase mutants from the mesophilic bacterium Clostridium beijerinckii: contribution of salt bridging" @default.
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• W2005539780 cites W1482938752 @default.
• W2005539780 cites W1521025514 @default.
• W2005539780 cites W1529159985 @default.
• W2005539780 cites W1539796472 @default.
• W2005539780 cites W1757079284 @default.
• W2005539780 cites W1875021313 @default.
• W2005539780 cites W1886064066 @default.
• W2005539780 cites W1963858501 @default.
• W2005539780 cites W1970779799 @default.
• W2005539780 cites W1972946273 @default.
• W2005539780 cites W1980022131 @default.
• W2005539780 cites W1980996961 @default.
• W2005539780 cites W1983659508 @default.
• W2005539780 cites W1984212866 @default.
• W2005539780 cites W1985842673 @default.
• W2005539780 cites W1986191025 @default.
• W2005539780 cites W1986550844 @default.
• W2005539780 cites W1987268759 @default.
• W2005539780 cites W1995017064 @default.
• W2005539780 cites W1998865695 @default.
• W2005539780 cites W1998902553 @default.
• W2005539780 cites W1999147504 @default.
• W2005539780 cites W2000980755 @default.
• W2005539780 cites W2003955698 @default.
• W2005539780 cites W2011276710 @default.
• W2005539780 cites W2015279243 @default.
• W2005539780 cites W2015536590 @default.
• W2005539780 cites W2018289835 @default.
• W2005539780 cites W2020947557 @default.
• W2005539780 cites W2021534482 @default.
• W2005539780 cites W2022081324 @default.
• W2005539780 cites W2025389130 @default.
• W2005539780 cites W2026911129 @default.
• W2005539780 cites W2028854152 @default.
• W2005539780 cites W2031778321 @default.
• W2005539780 cites W2033756350 @default.
• W2005539780 cites W2035407823 @default.
• W2005539780 cites W2035482680 @default.
• W2005539780 cites W2036931150 @default.
• W2005539780 cites W2040351161 @default.
• W2005539780 cites W2040403917 @default.
• W2005539780 cites W2040407044 @default.
• W2005539780 cites W2044149388 @default.
• W2005539780 cites W2047841570 @default.
• W2005539780 cites W2048411275 @default.
• W2005539780 cites W2048591080 @default.
• W2005539780 cites W2052163477 @default.
• W2005539780 cites W2052380879 @default.
• W2005539780 cites W2054403251 @default.
• W2005539780 cites W2057443486 @default.
• W2005539780 cites W2058040937 @default.
• W2005539780 cites W2062129432 @default.
• W2005539780 cites W2070184351 @default.
• W2005539780 cites W2071168617 @default.
• W2005539780 cites W2077141405 @default.
• W2005539780 cites W2092784580 @default.
• W2005539780 cites W2094919836 @default.
• W2005539780 cites W2095375426 @default.
• W2005539780 cites W2095805596 @default.
• W2005539780 cites W2096520520 @default.
• W2005539780 cites W2099454812 @default.
• W2005539780 cites W2100578005 @default.
• W2005539780 cites W2100837269 @default.
• W2005539780 cites W2105590412 @default.
• W2005539780 cites W2106320506 @default.
• W2005539780 cites W2107543794 @default.
• W2005539780 cites W2125097548 @default.
• W2005539780 cites W2128635872 @default.
• W2005539780 cites W2136979062 @default.
• W2005539780 cites W2137189075 @default.
• W2005539780 cites W2145189739 @default.
• W2005539780 cites W2147694156 @default.
• W2005539780 cites W2153195964 @default.
• W2005539780 cites W2154665416 @default.
• W2005539780 cites W2159422614 @default.
• W2005539780 cites W2160085559 @default.
• W2005539780 cites W2171807422 @default.
• W2005539780 cites W4293247451 @default.
• W2005539780 doi "https://doi.org/10.1110/ps.0222102" @default.
• W2005539780 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2373725" @default.
• W2005539780 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12381840" @default.
• W2005539780 hasPublicationYear "2002" @default.
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