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• W2005597971 abstract "Intercellular adhesion strengthening, a phenomenon that compromises the texture and the edible quality of potatoes (Solanum tuberosum L.), has been induced reproducibly by exposure to low-pH acetic acid solutions under tissue culture conditions. The resulting parenchyma tissues have been examined by solid-state nuclear magnetic resonance (NMR) in order to characterize the biopolymer(s) thought to be associated with this syndrome. Cross polarization−magic angle spinning (CPMAS) 13C NMR has been used to establish the presence of a polyphenol−suberin-like aromatic−aliphatic polyester within an abundant cell wall polysaccharide matrix in potato tubers that exhibit hardening due to strengthened intercellular adhesion. Dipolar dephasing and CP chemical shift anisotropy experiments suggest that the aromatic domain is composed primarily of guaiacyl and sinapyl groups. Two-dimensional wide-line separation experiments show that the biopolymer associated with parenchyma hardening contains rigid polysaccharide cell walls and mobile aliphatic long-chain fatty acids; 1H spin diffusion experiments show that these flexible aliphatic chains are proximal to both the phenolics and a subpopulation of the cell wall polysaccharides. Finally, high-resolution MAS NMR of parenchyma samples swelled in DMSO in conjunction with two-dimensional through-bond and through-space NMR spectroscopy provides evidence for covalent linkages among the polysaccharide, phenolic, and aliphatic domains of the intercellular adhesion-strengthening biopolymer in potato parenchyma tissue." @default.
• W2005597971 created "2016-06-24" @default.
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• W2005597971 date "2006-02-22" @default.
• W2005597971 modified "2023-09-26" @default.
• W2005597971 title "Magic-Angle Spinning NMR Studies of Cell Wall Bound Aromatic−Aliphatic Biopolyesters Associated with Strengthening of Intercellular Adhesion in Potato (<i>Solanum tuberosum </i>L.) Tuber Parenchyma" @default.
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• W2005597971 doi "https://doi.org/10.1021/bm050812t" @default.
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