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- W2005617158 abstract "A DNA sequence encoding rat neurorial NO synthase (nNOS) was isolated and cloned into the baculovirus expression vector pVL1393 to generate pVLRBNOS. Transfection of Spodoptera frugiperda Sf-21 cells with the construct pVLRBNOS resulted in the synthesis of high levels of neuronal NO synthase. Analysis of the expression pattern revealed soluble, enzymatically active NO synthase in the cytoplasm of cell extracts. Active enzyme could also be purified from culture supernatants using 2′-5′ ADP sepharose affinity chromatography. This enzyme was recognised by antibodies to the native nNOS and showed a similar degree of inhibition by arginine analogs as the native nNOS. The majority of the NOS synthesised had accumulated as insoluble inclusion-body material. The purification of recombinant nNOS from insect cells should facilitate characterisation of neuronal NO synthase." @default.
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- W2005617158 date "1993-11-01" @default.
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- W2005617158 title "Cloning and Expression of a Rat Neuronal Nitric Oxide Synthase Coding Sequence in a Baculovirus/Insect Cell System" @default.
- W2005617158 doi "https://doi.org/10.1006/bbrc.1993.2419" @default.
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