FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005643802> ?p ?o ?g. }

• W2005643802 endingPage "23" @default.
• W2005643802 startingPage "8" @default.
• W2005643802 abstract "Transformation of preneoplastic epidermal JB6 cells with tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) is an in vitro model of late-stage tumor promotion. Osteopontin (OPN) is a secreted, adhesive protein that is highly expressed in JB6 cells with TPA treatment, and its expression persists for at least 4 days, which is the time required for subsequent expression of transformed phenotype. These observations suggest that OPN may play a role in promoting JB6 cell transformation. To function in transformation of JB6 cells, OPN must bind to the surface of the JB6 cell and subsequently signal within the cell. Therefore, we investigated whether JB6 cells adhere to OPN and, if so, to which surface receptors. TPA-treated JB6 cells had significantly (P < 0.05) increased adherence to OPN compared with dimethylsulfoxide-treated control cells. Enhanced attachment of JB6 cells to OPN was also observed after treatment with another tumor promoter phorbol dibutyrate but not with nontumor promoters (phorbol and 1α,25-dihydroxyvitamin D3), suggesting that tumor promoters specifically modulate attachment to OPN. The argininylglycylaspartic acid (RGD) cell-binding region of OPN mediates attachment of TPA-treated JB6 cells because RGD, but not argininylglycylglutamic acid (RGE), peptides inhibited adherence of these cells to OPN in a dose-dependent manner. Flow cytometric analyses, blocking adhesion assay using anti-αv antibody, and co-immunoprecipitation assay all indicated that TPA-treated cells had similar levels of αv and β5 but decreased levels of β1 compared with untreated cells and that cell adhesion to OPN is most likely mediated through the αvβ5. Furthermore, calphostin C, a specific protein kinase C (PKC) inhibitor, decreased TPA-treated JB6 cell adhesion to OPN by 50%, suggesting that TPA increased integrin affinity or avidity for OPN through a PKC-mediated pathway. Collectively, these results indicate that transforming JB6 cells adhere to OPN through its RGD sequence. The most likely OPN receptor is the αvβ5 integrin, which increases the affinity or avidity for OPN through a PKC-dependent pathway rather than increasing the number of receptors. J. Cell. Biochem. 78:8–23, 2000. © 2000 Wiley-Liss, Inc." @default.
• W2005643802 created "2016-06-24" @default.
• W2005643802 creator A5032502592 @default.
• W2005643802 creator A5065205944 @default.
• W2005643802 date "2000-07-01" @default.
• W2005643802 modified "2023-10-13" @default.
• W2005643802 title "Transforming JB6 cells exhibit enhanced integrin-mediated adhesion to osteopontin" @default.
• W2005643802 cites W1499024571 @default.
• W2005643802 cites W1499636416 @default.
• W2005643802 cites W1511542029 @default.
• W2005643802 cites W1529319601 @default.
• W2005643802 cites W1534534898 @default.
• W2005643802 cites W1575698723 @default.
• W2005643802 cites W1649140242 @default.
• W2005643802 cites W1839610592 @default.
• W2005643802 cites W1939982746 @default.
• W2005643802 cites W1966723290 @default.
• W2005643802 cites W1968051889 @default.
• W2005643802 cites W1969361987 @default.
• W2005643802 cites W1969847469 @default.
• W2005643802 cites W1983909759 @default.
• W2005643802 cites W1984243344 @default.
• W2005643802 cites W1988869074 @default.
• W2005643802 cites W1991953529 @default.
• W2005643802 cites W1994724799 @default.
• W2005643802 cites W1997697459 @default.
• W2005643802 cites W2000817261 @default.
• W2005643802 cites W2009379675 @default.
• W2005643802 cites W2013198332 @default.
• W2005643802 cites W2021653786 @default.
• W2005643802 cites W2046086515 @default.
• W2005643802 cites W2046983903 @default.
• W2005643802 cites W2049031103 @default.
• W2005643802 cites W2058547055 @default.
• W2005643802 cites W2064484362 @default.
• W2005643802 cites W2068362124 @default.
• W2005643802 cites W2069246523 @default.
• W2005643802 cites W2072917762 @default.
• W2005643802 cites W2077336026 @default.
• W2005643802 cites W2077569218 @default.
• W2005643802 cites W2079312655 @default.
• W2005643802 cites W2092085533 @default.
• W2005643802 cites W2166179228 @default.
• W2005643802 cites W2179433498 @default.
• W2005643802 cites W2412635390 @default.
• W2005643802 doi "https://doi.org/10.1002/(sici)1097-4644(20000701)78:1<8::aid-jcb2>3.0.co;2-s" @default.
• W2005643802 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10797562" @default.
• W2005643802 hasPublicationYear "2000" @default.
• W2005643802 type Work @default.
• W2005643802 sameAs 2005643802 @default.
• W2005643802 citedByCount "18" @default.
• W2005643802 countsByYear W20056438022013 @default.
• W2005643802 countsByYear W20056438022014 @default.
• W2005643802 countsByYear W20056438022019 @default.
• W2005643802 crossrefType "journal-article" @default.
• W2005643802 hasAuthorship W2005643802A5032502592 @default.
• W2005643802 hasAuthorship W2005643802A5065205944 @default.
• W2005643802 hasConcept C1491633281 @default.
• W2005643802 hasConcept C153911025 @default.
• W2005643802 hasConcept C185592680 @default.
• W2005643802 hasConcept C195687474 @default.
• W2005643802 hasConcept C195794163 @default.
• W2005643802 hasConcept C203014093 @default.
• W2005643802 hasConcept C2780043322 @default.
• W2005643802 hasConcept C2780804394 @default.
• W2005643802 hasConcept C54355233 @default.
• W2005643802 hasConcept C55493867 @default.
• W2005643802 hasConcept C62478195 @default.
• W2005643802 hasConcept C81885089 @default.
• W2005643802 hasConcept C85789140 @default.
• W2005643802 hasConcept C86492073 @default.
• W2005643802 hasConcept C86803240 @default.
• W2005643802 hasConcept C95444343 @default.
• W2005643802 hasConceptScore W2005643802C1491633281 @default.
• W2005643802 hasConceptScore W2005643802C153911025 @default.
• W2005643802 hasConceptScore W2005643802C185592680 @default.
• W2005643802 hasConceptScore W2005643802C195687474 @default.
• W2005643802 hasConceptScore W2005643802C195794163 @default.
• W2005643802 hasConceptScore W2005643802C203014093 @default.
• W2005643802 hasConceptScore W2005643802C2780043322 @default.
• W2005643802 hasConceptScore W2005643802C2780804394 @default.
• W2005643802 hasConceptScore W2005643802C54355233 @default.
• W2005643802 hasConceptScore W2005643802C55493867 @default.
• W2005643802 hasConceptScore W2005643802C62478195 @default.
• W2005643802 hasConceptScore W2005643802C81885089 @default.
• W2005643802 hasConceptScore W2005643802C85789140 @default.
• W2005643802 hasConceptScore W2005643802C86492073 @default.
• W2005643802 hasConceptScore W2005643802C86803240 @default.
• W2005643802 hasConceptScore W2005643802C95444343 @default.
• W2005643802 hasIssue "1" @default.
• W2005643802 hasLocation W20056438021 @default.
• W2005643802 hasLocation W20056438022 @default.
• W2005643802 hasOpenAccess W2005643802 @default.
• W2005643802 hasPrimaryLocation W20056438021 @default.
• W2005643802 hasRelatedWork W1763024686 @default.
• W2005643802 hasRelatedWork W1927751645 @default.
• W2005643802 hasRelatedWork W2041595484 @default.
• W2005643802 hasRelatedWork W2068491864 @default.

• next