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- W2005653607 abstract "Polyclonal antibodies were generated against the bacteriocin enterocin B by conjugating enterocin B (ENT) to bovine serum albumin (BSA) through a bifunctional crosslinking agent. Rabbits immunized with the ENT-BSA conjugates developed antibodies specific for enterocin B. Non-competive indirect (NCI) ELISAs were able to detect the presence of enterocin B in the supernatant of a producer strain and from a partially-purified sample. Competitive indirect (CI) ELISAs demonstrated concentrations of enterocin B ranging from 400-1600 ng ml−1 were required for 50% antibody binding inhibition. An immunoaffinity chromatography column was developed by linking the antibodies to CNBr-activated Sepahrose 4B. The purification method yielded pure enterocin; however, the harsh elution conditions did not render a regeneratable column." @default.
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- W2005653607 date "2001-12-01" @default.
- W2005653607 modified "2023-09-25" @default.
- W2005653607 title "Production of Antibodies against Enterocin B for Immunological Detection and Purification Purposes" @default.
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- W2005653607 doi "https://doi.org/10.1080/09540100120094492" @default.
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