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- W2005655327 abstract "Abstract Rationale Specific IgE antibodies have been detected in hexamethylene diisocyanate (HDI) exposed workers by different investigators using different antigens. Low affinity anti-hapten antibodies are less likely than high affinity antibodies to react with poorly substituted hapten-protein antigen conjugates. We evaluated HDI-specific IgE antibody results in ELISA tests using conjugates with either low (HDI 3 :HSA) or high (HDI 23 :HSA) hapten:protein molar ratio. Methods HDI-exposed workers (n=74) were evaluated for diisocyanate asthma by specific inhalation challenge (SIC) and for IgE antibodies by ELISA tests using plates coated with either HDI 3 or HDI 23 antigen. ELISA readings were taken when a + control serum achieved an A 405 of 0.60 against the HDI 23 antigen. Relative affinity of IgE antibody was determined as the A 405 ratio in tests of the 2 conjugates (HDI 3 :HDI 23 ). Results Relative affinities of IgE antibodies varied from 0.07 to 1.08. The frequency of IgE antibody was 18.9% with HDI 23 antigen compared to 9.4% with HDI 3 . IgE was found in 30.4% of SIC+ workers and 13.7% of SIC- workers with HDI 23 ; 21% and 4% with HDI 3 . Test sensitivity was 58.3% with HDI 23 versus 41% for HDI 3 , and test specificity was 78% and 93.7% for HDI 23 and HDI 3 , respectively. Conclusions HDI:HSA antigen with a high molar ratio detects both low and high affinity specific IgE antibodies but with reduced test specificity. Affirmation of conformance to principles of Declaration of Helsinki of the World Medical Association; Funding provided by NIOSH grant RO1 OH 03519 and FIS/Fulbright Scholarship Grant awarded to P Campo." @default.
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- W2005655327 date "2004-02-01" @default.
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- W2005655327 title "HDI-specific IgE antibody results are influenced by relative antibody affinity*1" @default.
- W2005655327 doi "https://doi.org/10.1016/j.jaci.2003.12.185" @default.
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