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• W2005679966 abstract "Red beet (Beta vulgaris L.) tonoplast membrane vesicles and [14C]trans-cinnamic acid-glutathione were used to study the vacuolar transport of phenylpropanoid-glutathione conjugates which are formed in peroxidase-mediated reactions. It was determined that the uptake of [14C]trans-cinnamic acid-glutathione into the tonoplast membrane vesicles was MgATP dependent and was 10-fold faster than the uptake of non-conjugated [14C]trans-cinnamic acid. Uptake of the conjugate in the presence of MgATP was not dependent on a trans-tonoplast H+-electrochemical gradient, because uptake was not affected by the addition of NH4Cl (1 mM; 0% inhibition) and was only slightly affected by gramicidin-D (5 μM; 14% inhibition). Uptake of the conjugate was inhibited 92% by the addition of vanadate (1 mM) and 71% by the addition of the model substrate S-(2,4-dinitrophenyl) glutathione (500 μM). Uptake did not occur when a nonhydrolyzable analog of ATP was used in place of MgATP. The calculated Km and Vmax values for uptake were 142 μM and 5.95 nmol mg−1 min−1, respectively. Based on these results, phenylpropanoid-glutathione conjugates formed in peroxidase-mediated reactions appear to be transported into the vacuole by the glutathione S-conjugate pump(s) located in the tonoplast membrane." @default.
• W2005679966 created "2016-06-24" @default.
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• W2005679966 date "2000-02-01" @default.
• W2005679966 modified "2023-09-23" @default.
• W2005679966 title "Vacuolar transport of the glutathione conjugate of trans-cinnamic acid" @default.
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• W2005679966 doi "https://doi.org/10.1016/s0031-9422(99)00563-4" @default.
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