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• W2005685079 abstract "A 16‐year‐old schoolgirl presented with a complaint of enlarging erythematous and mildly pruritic patches located on the buttocks of 2 years’ duration. She had been treated with a potent topical corticosteroid for many months without apparent benefit. She was in otherwise good health. She denied a history of hair infection, previous eruptions, asthma, or hay fever. No one in her family or friends had similar lesions. She had no pets in her house. Examination revealed the presence of two erythematous and slightly indurated plaques measuring approximately 16 cm × 9 cm and 6 cm × 11 cm in diameter on her right and left buttocks, respectively ( Fig. 1 ). Similar progressively enlarging lesions were present on the dorsum of her right hand, extending to the extensor aspect of the forearm and to the lateral, inner, palmar, and extensor aspects of the second, third, and fourth fingers, and on the dorsum of her left hand up to the flexor aspect of the wrist. A few erythematous papules clustering around these plaques in a satellite fashion were also detectable. Her scalp, pubic, axillary, eyebrow, and eyelash hair appeared normal with no concretions or soft, lightly pigmented, white to light‐brown, loosely attached, irregularly spaced nodules. There was no regional or generalized lymphadenopathy or hepatosplenomegaly. Routine laboratory investigations, human immunodeficiency virus (HIV) testing, and standard patch tests were negative. Erythematous and slightly indurated plaques on the right and left buttocks image A skin biopsy specimen from the right buttock showed parakeratosis and acanthosis in the epidermis, with some neutrophils within the parakeratotic crust, and a mild inflammatory infiltrate in the dermis. In the stratum corneum, fungal hyphae were detectable by periodic acid–Schiff (PAS) staining ( Fig. 2 ). Direct microscopic examination with 15% potassium hydroxide (KOH) preparation of scale showed pseudohyphae. Two mycotic cultures were obtained: the first on Sabouraud dextrose agar (SDA) medium (2% glucose, 1% mycologic peptone, 0.5% yeast extract, 1.5% agar; Oxoid Ltd, Basingstoke, UK) supplemented with chloramphenicol (Sigma, St. Louis, MO, USA) and cycloheximide (Sigma, St. Louis, MO, USA) incubated for 2 weeks at 25 °C; the second on SDA medium supplemented with chloramphenicol and gentamycin (Oxoid Ltd, Basingstoke, UK) incubated for 2 weeks at 30 °C ( Fig. 3 ). Skin biopsy from a tissue specimen in which fungal hyphae were detectable in the stratum corneum (periodic acid–Schiff, ×40) image Mycologic culture of isolated Trichosporon sp. (Sabouraud dextrose agar medium with chloramphenicol and gentamycin) image The cultures yielded cream‐colored, wrinkled colonies, microscopic examination (with lactophenol cotton blue) of which showed true hyphae, pseudohyphae, a few blastoconidia, and rectangular arthroconidia typical of Trichosporon sp. ( Fig. 4 ). These colonies were identified as Trichosporon a sahii by cornmeal agar medium with the addition of Tween‐80, according to the Dalmau plate technique ( Fig. 5 ), and on API 20C AUX assimilation assay (BioMerieux, Inc., Lyon, France). Features of Trichosporon sp. at microscopic examination (lactophenol cotton blue stain, ×100) image Morphologic characteristics of Trichosporon asahii (cornmeal agar medium with the addition of Tween‐80, ×40) image The patient was given oral itraconazole (200 mg/day) and topical tioconazole cream for 6 weeks, which produced complete clinical and mycologic cure ( Fig. 6 ). No relapse was observed at the 6‐month follow‐up. Clinical aspect after treatment image" @default.
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• W2005685079 date "2006-12-01" @default.
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• W2005685079 title "Superficial cutaneous Trichosporon asahii infection in an immunocompetent host" @default.
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• W2005685079 doi "https://doi.org/10.1111/j.1365-4632.2006.02700.x" @default.
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