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- W2005686908 abstract "A protein-free medium was developed which supports the growth of human×human hybridomas derived from the fusion partner KR-4. This medium consists of a 1:1 mixture of Iscove's modified Dulbecco's medium and Ham's F-12 medium supplemented with 300 μM ethanolamine. The addition of ethanolamine was essential to maintain cell viability and proliferation. Phosphoethanolamine could not substitute for ethanolamine. Although the maximal cell density achieved in protein-free medium was lower than that in medium supplemented with albumin and transferrin, immunoglobulin production was still supported. This protein-free medium may be useful for large-scale cultivation and for metabolic and immunologic studies of human hybridomas." @default.
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- W2005686908 date "1987-02-01" @default.
- W2005686908 modified "2023-09-26" @default.
- W2005686908 title "Growth of human × human hybridomas in protein-free medium supplemented with ethanolamine" @default.
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- W2005686908 doi "https://doi.org/10.1016/0022-1759(87)90101-3" @default.
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