FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005694414> ?p ?o ?g. }

• W2005694414 abstract "Glutathione (GSH), the major cellular protectant against reactive oxygen and nitrogen species, is compartmentalized in a cytosolic (c) and a mitochondrial (mt) pool. We investigated how c-GSH and mt-GSH are differentially affected by endogenously produced nitric oxide (NO). Microglial cell line (N9) cultures were immunostimulated with lipopolysaccharide/interferon-gamma to elicit the inducible isoform of NO synthase (iNOS). Despite a significant reduction in total GSH, the mt-GSH remained nearly unaffected by iNOS-mediated NO production. To investigate possible consequences of GSH depletion on the mitochondrial membrane potential, we used buthionine sulfoximine (BSO) to reduce separately the c-GSH, whereas ethacrynic acid (EA) was applied to deplete both mt-GSH and c-GSH. The mitochondrial membrane potential was more vulnerable to NO exposure in EA-pretreated cultures than in BSO-pretreated cultures, indicated by a potentiated release of tetramethylrhodamine from mitochondria into the cytosol. To relate the EA-mediated decrease in mitochondrial membrane potential to the oxidant buildup after GSH depletion, we loaded the cells with the oxidant-sensitive fluorochrome 2',7'-dihydrodichlorofluorescein (DCF) diacetate. EA treatment caused an increase in DCF fluorescence over time that was potentiated when the iNOS expression was stimulated. Inhibition of NO production abolished this effect. We conclude that endogenous NO production in microglial cells does not compromise the mt-GSH pool which, in turn, might explain the ability of these cells to combat high-output NO production." @default.
• W2005694414 created "2016-06-24" @default.
• W2005694414 creator A5005378637 @default.
• W2005694414 creator A5025782417 @default.
• W2005694414 creator A5040385559 @default.
• W2005694414 creator A5065080624 @default.
• W2005694414 date "2003-02-01" @default.
• W2005694414 modified "2023-10-14" @default.
• W2005694414 title "Cytosolic and mitochondrial glutathione in microglial cells are differentially affected by oxidative/nitrosative stress" @default.
• W2005694414 cites W1534977663 @default.
• W2005694414 cites W1545799264 @default.
• W2005694414 cites W1581740874 @default.
• W2005694414 cites W1699447737 @default.
• W2005694414 cites W1791420399 @default.
• W2005694414 cites W1972956620 @default.
• W2005694414 cites W1976180551 @default.
• W2005694414 cites W1990798856 @default.
• W2005694414 cites W1997766296 @default.
• W2005694414 cites W1999098395 @default.
• W2005694414 cites W2003663428 @default.
• W2005694414 cites W2018796723 @default.
• W2005694414 cites W2020034315 @default.
• W2005694414 cites W2023235828 @default.
• W2005694414 cites W2025680138 @default.
• W2005694414 cites W2048946571 @default.
• W2005694414 cites W2049453495 @default.
• W2005694414 cites W2051908396 @default.
• W2005694414 cites W2062753450 @default.
• W2005694414 cites W2067524848 @default.
• W2005694414 cites W2069594598 @default.
• W2005694414 cites W2082443643 @default.
• W2005694414 cites W2085226483 @default.
• W2005694414 cites W2090816846 @default.
• W2005694414 cites W2093243893 @default.
• W2005694414 cites W2093384103 @default.
• W2005694414 cites W2095685790 @default.
• W2005694414 cites W2099837366 @default.
• W2005694414 cites W2144361706 @default.
• W2005694414 cites W2164038145 @default.
• W2005694414 cites W2169714384 @default.
• W2005694414 cites W2410553234 @default.
• W2005694414 cites W28016325 @default.
• W2005694414 doi "https://doi.org/10.1016/s1089-8603(02)00146-5" @default.
• W2005694414 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12586540" @default.
• W2005694414 hasPublicationYear "2003" @default.
• W2005694414 type Work @default.
• W2005694414 sameAs 2005694414 @default.
• W2005694414 citedByCount "22" @default.
• W2005694414 countsByYear W20056944142012 @default.
• W2005694414 countsByYear W20056944142013 @default.
• W2005694414 countsByYear W20056944142014 @default.
• W2005694414 countsByYear W20056944142017 @default.
• W2005694414 countsByYear W20056944142020 @default.
• W2005694414 countsByYear W20056944142021 @default.
• W2005694414 crossrefType "journal-article" @default.
• W2005694414 hasAuthorship W2005694414A5005378637 @default.
• W2005694414 hasAuthorship W2005694414A5025782417 @default.
• W2005694414 hasAuthorship W2005694414A5040385559 @default.
• W2005694414 hasAuthorship W2005694414A5065080624 @default.
• W2005694414 hasConcept C153911025 @default.
• W2005694414 hasConcept C178790620 @default.
• W2005694414 hasConcept C181199279 @default.
• W2005694414 hasConcept C181911157 @default.
• W2005694414 hasConcept C185592680 @default.
• W2005694414 hasConcept C203014093 @default.
• W2005694414 hasConcept C2776151105 @default.
• W2005694414 hasConcept C2777476445 @default.
• W2005694414 hasConcept C2777622882 @default.
• W2005694414 hasConcept C2778754761 @default.
• W2005694414 hasConcept C2779525624 @default.
• W2005694414 hasConcept C28859421 @default.
• W2005694414 hasConcept C48349386 @default.
• W2005694414 hasConcept C519581460 @default.
• W2005694414 hasConcept C538909803 @default.
• W2005694414 hasConcept C55493867 @default.
• W2005694414 hasConcept C75385678 @default.
• W2005694414 hasConcept C86803240 @default.
• W2005694414 hasConcept C95444343 @default.
• W2005694414 hasConcept C98539663 @default.
• W2005694414 hasConceptScore W2005694414C153911025 @default.
• W2005694414 hasConceptScore W2005694414C178790620 @default.
• W2005694414 hasConceptScore W2005694414C181199279 @default.
• W2005694414 hasConceptScore W2005694414C181911157 @default.
• W2005694414 hasConceptScore W2005694414C185592680 @default.
• W2005694414 hasConceptScore W2005694414C203014093 @default.
• W2005694414 hasConceptScore W2005694414C2776151105 @default.
• W2005694414 hasConceptScore W2005694414C2777476445 @default.
• W2005694414 hasConceptScore W2005694414C2777622882 @default.
• W2005694414 hasConceptScore W2005694414C2778754761 @default.
• W2005694414 hasConceptScore W2005694414C2779525624 @default.
• W2005694414 hasConceptScore W2005694414C28859421 @default.
• W2005694414 hasConceptScore W2005694414C48349386 @default.
• W2005694414 hasConceptScore W2005694414C519581460 @default.
• W2005694414 hasConceptScore W2005694414C538909803 @default.
• W2005694414 hasConceptScore W2005694414C55493867 @default.
• W2005694414 hasConceptScore W2005694414C75385678 @default.
• W2005694414 hasConceptScore W2005694414C86803240 @default.
• W2005694414 hasConceptScore W2005694414C95444343 @default.
• W2005694414 hasConceptScore W2005694414C98539663 @default.
• W2005694414 hasLocation W20056944141 @default.

• next