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- W2005697451 abstract "Plasma membranes from Candida tropicalis grown on glucose or hexadecane were isolated using a method based on the difference in surface charge of mitochondria and plasma membranes. After mechanical disruption of the cells, a fraction consisting of mitochondrial and plasma membrane vesicles was obtained by differential centrifugation. Subsequently the mitochondria were separated from the plasma membrane vesicles by aggregation of the mitochondria at a pH corresponding to their isoelectric point. Additional purification of the isolated plasma membrane vesicles was achieved by osmolysis. Surface charge densities of mitochondria and plasma membranes were determined and showed substrate-dependent differences. The isolated plasma membranes were morphologically characterized by electron microscopy and, as a marker enzyme, the activity of Mg2+-dependent ATPase was determine. By checking for three mitochondrial marker enzymes the plasma membrane fractions were estimated to be 94% pure with regard to mitochondrial contamination." @default.
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- W2005697451 date "1978-10-01" @default.
- W2005697451 modified "2023-10-18" @default.
- W2005697451 title "Plasma membranes from candida tropicalis grown on glucose or hexadecane. I. Isolation, identification and purification" @default.
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- W2005697451 doi "https://doi.org/10.1016/0005-2736(78)90160-8" @default.
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