FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2005759040> ?p ?o ?g. }

• W2005759040 endingPage "717" @default.
• W2005759040 startingPage "707" @default.
• W2005759040 abstract "Evidence suggests that chromaffin cells employ separate mechanisms for evoked endocytosis and granule recycling when stimulated at basal (approximately 0.5 Hz) and stress-activated (approximately 15 Hz) rates. Previous studies have focused mainly on elucidating the cellular mechanisms responsible for membrane recycling under conditions similar to the stress-activated state and indicate a clathrin/dephosphin-mediated retrieval via coated pits. However, the mechanism for membrane internalisation at basal stimulus intensity remains largely unexplored. We electrically stimulated chromaffin cells in adrenal tissue slices at the sympathetic basal firing rate and measured cell capacitance in the perforated voltage clamp configuration. A new method for the separation of non-secretory from secretory cell capacitance signals is presented. Simultaneous catecholamine release was measured electrochemically to isolate the exocytic from endocytic components of the capacitance responses. Using this approach we demonstrate that firing patterns that mimic basal sympathetic input results in rapid and graded membrane retrieval. We show that block of the calcium-mediated protein phosphatase 2B, a common step in clathrin-mediated processes, did not alter endocytosis elicited at basal firing levels. We further blocked clathrin-mediated retrieval with a clathrin/dephosphin-disrupting peptide (PP-19) and found endocytosis to be blocked at 15 Hz stimulation but complete and indistinguishable from control cells at 0.5 Hz stimulation. Lastly, pharmacological treatments show that conventional isoforms of protein kinase C (cPKC) are required for the 0.5 Hz-evoked retrieval mechanism. From these data we conclude that unlike endocytosis evoked under stress conditions, basal firing activity results in a clathrin-independent rapid membrane retrieval mediated through conventional isoforms of PKC." @default.
• W2005759040 created "2016-06-24" @default.
• W2005759040 creator A5026127536 @default.
• W2005759040 creator A5049285409 @default.
• W2005759040 date "2003-12-01" @default.
• W2005759040 modified "2023-10-14" @default.
• W2005759040 title "Low Frequency Stimulation of Mouse Adrenal Slices Reveals a Clathrin‐Independent, Protein Kinase C‐Mediated Endocytic Mechanism" @default.
• W2005759040 cites W1523560961 @default.
• W2005759040 cites W1571965370 @default.
• W2005759040 cites W1602730687 @default.
• W2005759040 cites W1773128106 @default.
• W2005759040 cites W1920398807 @default.
• W2005759040 cites W1964008139 @default.
• W2005759040 cites W1973320665 @default.
• W2005759040 cites W1978390881 @default.
• W2005759040 cites W1982474924 @default.
• W2005759040 cites W1988867376 @default.
• W2005759040 cites W1990087708 @default.
• W2005759040 cites W1995408976 @default.
• W2005759040 cites W2003380714 @default.
• W2005759040 cites W2007461061 @default.
• W2005759040 cites W2008574946 @default.
• W2005759040 cites W2018264391 @default.
• W2005759040 cites W2024272753 @default.
• W2005759040 cites W2024784822 @default.
• W2005759040 cites W2026005895 @default.
• W2005759040 cites W2026740840 @default.
• W2005759040 cites W2028130617 @default.
• W2005759040 cites W2028189987 @default.
• W2005759040 cites W2030387038 @default.
• W2005759040 cites W2031521834 @default.
• W2005759040 cites W2033021180 @default.
• W2005759040 cites W2035149692 @default.
• W2005759040 cites W2035336615 @default.
• W2005759040 cites W2035379262 @default.
• W2005759040 cites W2047614532 @default.
• W2005759040 cites W2049228395 @default.
• W2005759040 cites W2052741683 @default.
• W2005759040 cites W2055672024 @default.
• W2005759040 cites W2067454609 @default.
• W2005759040 cites W2067904303 @default.
• W2005759040 cites W2080954023 @default.
• W2005759040 cites W2089657872 @default.
• W2005759040 cites W2090363261 @default.
• W2005759040 cites W2095341074 @default.
• W2005759040 cites W2095342717 @default.
• W2005759040 cites W2103317206 @default.
• W2005759040 cites W2111947024 @default.
• W2005759040 cites W2112393819 @default.
• W2005759040 cites W2116025461 @default.
• W2005759040 cites W2126730087 @default.
• W2005759040 cites W2135374394 @default.
• W2005759040 cites W2141022591 @default.
• W2005759040 cites W2146529473 @default.
• W2005759040 cites W2158131682 @default.
• W2005759040 cites W2165328088 @default.
• W2005759040 cites W2167888543 @default.
• W2005759040 cites W2167980709 @default.
• W2005759040 cites W2171405755 @default.
• W2005759040 cites W2172095199 @default.
• W2005759040 cites W2336231253 @default.
• W2005759040 cites W2482902567 @default.
• W2005759040 cites W2977752530 @default.
• W2005759040 cites W39219210 @default.
• W2005759040 doi "https://doi.org/10.1113/jphysiol.2003.053918" @default.
• W2005759040 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2343636" @default.
• W2005759040 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14500763" @default.
• W2005759040 hasPublicationYear "2003" @default.
• W2005759040 type Work @default.
• W2005759040 sameAs 2005759040 @default.
• W2005759040 citedByCount "42" @default.
• W2005759040 countsByYear W20057590402012 @default.
• W2005759040 countsByYear W20057590402013 @default.
• W2005759040 countsByYear W20057590402014 @default.
• W2005759040 countsByYear W20057590402015 @default.
• W2005759040 countsByYear W20057590402016 @default.
• W2005759040 countsByYear W20057590402017 @default.
• W2005759040 countsByYear W20057590402021 @default.
• W2005759040 countsByYear W20057590402022 @default.
• W2005759040 crossrefType "journal-article" @default.
• W2005759040 hasAuthorship W2005759040A5026127536 @default.
• W2005759040 hasAuthorship W2005759040A5049285409 @default.
• W2005759040 hasBestOaLocation W20057590402 @default.
• W2005759040 hasConcept C12554922 @default.
• W2005759040 hasConcept C134018914 @default.
• W2005759040 hasConcept C1491633281 @default.
• W2005759040 hasConcept C175969161 @default.
• W2005759040 hasConcept C24998067 @default.
• W2005759040 hasConcept C28005876 @default.
• W2005759040 hasConcept C49039625 @default.
• W2005759040 hasConcept C55493867 @default.
• W2005759040 hasConcept C63162447 @default.
• W2005759040 hasConcept C79747257 @default.
• W2005759040 hasConcept C86803240 @default.
• W2005759040 hasConcept C95444343 @default.
• W2005759040 hasConceptScore W2005759040C12554922 @default.
• W2005759040 hasConceptScore W2005759040C134018914 @default.
• W2005759040 hasConceptScore W2005759040C1491633281 @default.

• next